LSI78Y mouse lymphoblastic leukemia cells normally require asparagine (ASN) for growth, but phenotypically stable ASN-independent cells are detectable at low fre quency by direct cloning, in ASN-free soft-agar medium, of cells from cultures that have been grown nonselectively in medium containing ASN. The rate of mutation in vitro to ASN independence was determined by the Luria-Delbruck fluctua...

Recombinant human phenylalanine hydroxylase (hPAH) expressed in Escherichia coli for 24 h at 28 degrees C has been found by two-dimensional electrophoresis to exist as a mixture of four to five molecular forms as a result of nonenzymatic deamidation of labile Asn residues. The multiple deamidations alter the functional properties of the enzyme including its affinity for l-phenylalanine and tetr...

Welcome to the 47th Annual Meeting of the American Society of Nephrology (ASN)—Kidney Week 2013. Thank you for helping to lead the fight against kidney disease in the United States and across the globe. As ASN President, I would like to thank you for the opportunity to lead this outstanding organization over the past year. I did it with the help of a remarkable and interactive ASN Council and t...

Reinforcement learning (RL) methods have proved to be successful in many simulated environments. The common approaches, however, are often too sample intensive to be applied directly in the real world. A promising approach to addressing this issue is to train an RL agent in a simulator and transfer the solution to the real environment. When a highfidelity simulator is available we would expect ...

Leadership style and work facilities are important factors in improving the performance of civil servants. is behavior or method used by a leader influencing thoughts, feelings, attitudes members subordinates. While supporting company's activities physical form, normal company activities, have relatively permanent period use provide benefits for future. This study aims to analyze effect leaders...

The magnitudes of the systematic biases involved in sample heterozygosity and sample genetic distances are evaluated, and formulae for obtaining unbiased estimates of average heterozygosity and genetic distance are developed. It is also shown that the number of individuals to be used for estimating average heterozygosity can be very small if a large number of loci are studied and the average he...

The Public Key Infrastructure using X.509 (PKIX) certificate format, and many associated formats, are expressed using ASN.1. The current ASN.1 modules conform to the 1988 version of ASN.1. This document updates those ASN.1 modules to conform to the 2002 version of ASN.1. There are no bits-on-the-wire changes to any of the formats; this is simply a change to the syntax.

The Public Key Infrastructure using X.509 (PKIX) certificate format, and many associated formats, are expressed using ASN.1. The current ASN.1 modules conform to the 1988 version of ASN.1. This document updates those ASN.1 modules to conform to the 2002 version of ASN.1. There are no bits-on-the-wire changes to any of the formats; this is simply a change to the syntax.

This paper proposes a database system based on ASN.1 (Abstract Syntax Notation One). Aiming at excluding the ambiguity of data representation in the heterogeneous computer environments, ASN.1 notation as well as its encoding rules is used in many advanced applications including those in communication networks. As the amount of data described by ASN.1 becomes very large, it is eagerly required t...