نتایج جستجو برای: autolysin gene

تعداد نتایج: 1141653  

Journal: :PLoS ONE 2008
Sean M. Rollins Amanda Peppercorn John S. Young Melissa Drysdale Andrea Baresch Margaret V. Bikowski David A. Ashford Conrad P. Quinn Martin Handfield Jeffrey D. Hillman C. Rick Lyons Theresa M. Koehler Stephen B. Calderwood Edward T. Ryan

In vivo induced antigen technology (IVIAT) is an immuno-screening technique that identifies bacterial antigens expressed during infection and not during standard in vitro culturing conditions. We applied IVIAT to Bacillus anthracis and identified PagA, seven members of a N-acetylmuramoyl-L-alanine amidase autolysin family, three P60 family lipoproteins, two transporters, spore cortex lytic prot...

Journal: :Indian journal of biochemistry & biophysics 2005
Xianghu Liu Xiaolong Xu Jiexia Chen Wenqi Liu Qingliang Liu

Acutolysin A, a protein isolated from the venom of Chinese Five-pace snake (Agkistrodon acutus) has shown marked hemorrhagic and proteolytic activities. In the present study, the effects of metal ions and an inhibitor EDTA on the fluorescence and function of autolysin A have been studied, by following fluorescence and activity measurements. Acutolysin A contains a Ca(2+)-binding site, which pro...

Journal: :Journal of bacteriology 1999
M Ramirez E Severina A Tomasz

The majority (591 of 791, or 76%) of Streptococcus pneumoniae clinical isolates examined showed the presence of two or more chromosomal SmaI fragments that hybridized with the lytA-specific DNA probe. Only one of these fragments, frequently having an approximate molecular size of 90 kb, was shown to carry the genetic determinant of the pneumococcal autolysin (N-acetylmuramic acid-L-alanine amid...

2010
Sebastian Zoll Bernhard Pätzold Martin Schlag Friedrich Götz Hubert Kalbacher Thilo Stehle

The major autolysins (Atl) of Staphylococcus epidermidis and S. aureus play an important role in cell separation, and their mutants are also attenuated in virulence. Therefore, autolysins represent a promising target for the development of new types of antibiotics. Here, we report the high-resolution structure of the catalytically active amidase domain AmiE (amidase S. epidermidis) from the maj...

Journal: :Microbiology 2014
Alistair J Standish Jonathan J Whittall Renato Morona

Tyrosine phosphorylation has long been recognized as a crucial post-translational regulatory mechanism in eukaryotes. However, only in the past decade has recognition been given to the crucial importance of bacterial tyrosine phosphorylation as an important regulatory feature of pathogenesis. This study describes the effect of tyrosine phosphorylation on the activity of a major virulence factor...

Journal: :Infection and immunity 1999
A M Whatmore S J King N C Doherty D Sturgeon N Chanter C G Dowson

Although often considered a strict human pathogen, Streptococcus pneumoniae has been reported to infect and cause pneumonia in horses, although the pathology appears restricted compared to that of human infections. Here we report on the molecular characterization of a group of S. pneumoniae isolates obtained from horses in England and Ireland. Despite being obtained from geographically distinct...

2011
Aurelia Delaune Olivier Poupel Adeline Mallet Yves-Marie Coic Tarek Msadek Sarah Dubrac

The WalKR two-component system is essential for viability of Staphylococcus aureus, a major pathogen. We have shown that WalKR acts as the master controller of peptidoglycan metabolism, yet none of the identified regulon genes explain its requirement for cell viability. Transmission electron micrographs revealed cell wall thickening and aberrant division septa in the absence of WalKR, suggestin...

Journal: :Journal of bacteriology 2015
Vincent Leung Dragana Ajdic Stephanie Koyanagi Céline M Lévesque

The presence of multidrug-tolerant persister cells within microbial populations has been implicated in the resiliency of bacterial survival against antibiotic treatments and is a major contributing factor in chronic infections. The mechanisms by which these phenotypic variants are formed have been linked to stress response pathways in various bacterial species, but many of these mechanisms rema...

2013
Yifan Xu Jens Kreth

Extracellular DNA (eDNA) is an important component of the biofilm matrix produced by many bacteria. In general, the release of eDNA is associated with the activity of muralytic enzymes leading to obvious cell lysis. In the Gram-positive oral commensal Streptococcus gordonii, eDNA release is dependent on pyruvate oxidase generated hydrogen peroxide (H2O2). Addition of H2O2 to cells grown under c...

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