An in vitro system was used to study DNA synthesis in lysates of Escherichia coli cells which had been grown in the presence of ethionine. Such lysates showed a reduced capacity to incorporate [3H]TTP into high-molecular-weight material. Activity could be restored by incubation with S-adenosyl methionine and ATP. S-adenosyl methionine-reactivated TTP incorporation required the presence of DNA p...

To cleave DNA, the Type III RM (restriction-modification) enzymes must communicate the relative orientation of two recognition sequences, which may be separated by many thousands of base pairs. This long-range interaction requires ATP hydrolysis by a helicase domain, and both active (DNA translocation) and passive (DNA sliding) modes of motion along DNA have been proposed. Potential roles for A...

pioglitazone (pg) is one of thiazolidinediones used for the treatment of type ii diabetes mellitus. some reports of its hepatotoxicity exist, but the mechanism of its hepatotoxicity is not well known. in the present study, the protective effect of some atp suppliers are investigated against mitochondrial toxicity of pg in isolated rat mitochondria. mitochondrial viability was investigated by mt...

background: the mitochondria are an important source of adenosine triphosphate (atp) production in pre-implantation embryo. therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and atp content. methods: the embryos at 2-pn, 4-cell and blastocyst stages were collected from the oviduct of st...

Escherichia coli cells treated with arsenate cannot tumble. The relationship between cellular adenosine 5'-triphosphate (ATP) level and the ability to tumble has been studied. (i) Cells incubated with arsenate completely lost their tumbling ability, and the cellular ATP level was decreased to less than 0.3 nmol/mg of protein. (ii) Incubation with 10 mM arsenate-1 mM phosphate reduced the cellul...

PURPOSE To develop an efficient 31 P magnetic resonance spectroscopy (MRS) method for measuring creatine kinase (CK) activity, adenosine triphosphate (ATP) synthesis, and motion dynamics in the human brain at 7 Tesla (T). METHODS Three band inversion modules differing in center frequency were used to induce magnetization transfer (MT) effect in three exchange pathways: (i) CK-mediated reactio...

To the Editor: Wang et al. (Dec. 21 issue)1 suggest that novel biomarkers in aggregate have little influence on the prediction of first cardiovascular events or death. We wonder whether there were sufficient analyses to warrant this conclusion. As described, there were only 68 “major” cardiovascular events in women and 101 in men. In the crucial multimarker analyses, a quarter of these events w...

Objective. To evaluate the prevalence of metabolic syndrome (MetS) according to the International Diabetes Federation (IDF) and Adult Treatment Panel (ATP) III criteria among working East African adults. Design. This cross-sectional study of 1,935 individuals (1,171 men and 764 women) was conducted among working adults in Addis Ababa, Ethiopia. The study was conducted in accordance with the STE...