plasma membrane h+-atpase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. to study the effect of nacl on the expression pattern of a gene encoding the plasma membrane h+-atpase, an experiment was carried out in a completely random design with three replications. a pair of specific primers was designed based on the sequence o...

OBJECTIVES To determine if maternal serum levels of 25(OH)D at 11-13 weeks' gestation are altered in pregnancies that subsequently deliver small for gestational age (SGA) neonates and whether the levels are related to placental function reflected in serum concentration of pregnancy-associated plasma protein-A (PAPP-A). METHODS Serum 25(OH)D and PAPP-A were measured at 11-13 weeks in 150 singl...

SNAP-25 is a component of the SNARE complex that is involved in membrane docking and fusion. Using a yeast two-hybrid screen, we identify a novel interaction between SNAP-25 and cytoplasmic Lek1 (cytLEK1), a protein previously demonstrated to associate with the microtubule network. The binding domains within each protein were defined by yeast two-hybrid, coimmunoprecipitation, and colocalizatio...

objective(s): adeno-associated virus type 2 (aav2) vectors are widely used for both experimental and clinical gene therapy. a recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. in this study, a fast and simple method is presented to generate aav2 vector helper plasmids encodin...

Beta 2 glycoprotein I (β2GPI) is a single chain 50 kDa highly glycosylated glycoprotein at an approximate concentration of 4 μM in cells. The abundance of this protein in plasma and its high state of preservation indicate the important role of this protein in mammalian. In addition, β2GPI has a particular structure in the fifth domain, and is categorized as the major antigen recognized by autoa...

The SecY complex associates with the ribosome to form a protein translocation channel in the bacterial plasma membrane. We have used cryo-electron microscopy and quantitative mass spectrometry to show that a nontranslating E. coli ribosome binds to a single SecY complex. The crystal structure of an archaeal SecY complex was then docked into the electron density maps. In the resulting model, two...

essential hypertension (eh) accounts for 90-95% of hypertension cases. eh, as a progressive cardiovascular syndrome arising from complex and interrelated etiologies, results from combined and interactive effects of genetic and environmental factors. the objective was to investigate the ace i/d and gnb3 c825t polymorphisms and both genotypes combined with seeking gene-gene interactions to furthe...