The reverse reaction of aspartate transcarbamylase in which phosphate or arsenate is first coupled to carbamyl aspartate, followed by elimination of aspartate, has been studied under conditions in which one product, aspartate, is removed. Aspartate is converted to oxalacetate by glutamate-oxalacetate transaminase, and the resulting oxalacetate is converted to malate by the NADH, NAD+ oxidoreduc...

Abstract Background The plant Culcasia angolensis (Araceae) has diverse ethnomedicinal uses, including the management of rheumatic pain, healing cuts, dislocations, and bruises. Despite its potential therapeutic toxicity profile not been evaluated. This study assessed sub-acute effects leaves extract (CAE). phytochemical determination CAE was conducted as per standard protocols. median lethal d...

Background Liver ischemia/reperfusion (I/R) injury is a complex and multifactorial pathophysiological process. It well recognized that the membrane permeability transition pore (mPTP) opening of mitochondria plays crucial role in cell death after I/R injury. Cyclophilin D (CypD) critical positive regulator mPTP. However, effect CypD on pathogenesis liver whether potential therapeutic target are...

The effects of stocking density on growth performance, serum biochemistry, digestive enzymes, immune response, and muscle quality largemouth bass (Micropterus salmoides) reared in nine in-pond raceway systems (IPRS, 22.0 m × 5.0 2.0 m) were studied. M. salmoides with initial an body weight 8.25 ± 0.51 g length 6.99 0.44 cm at 90.91 ind./m3 (low density, LSD), 113.63 (middle MSD), 136.36 (high H...

The simultaneous application of chemical modification of primary amines and the insertion of a lgF probe have been used to detect the nature and physical properties of the amino group influencing a selected region of the active center of the enzyme aspartate aminotransferase. The enzyme, carbamylated at the NH,-terminal alanine (transaminase’, produced from cyanate-treated holoenzyme) or at the...

Aspartate and glutamate react instantaneously with the pyridoxal form of the pig heart glutamic aspartic transaminase (1) to yield the corresponding keto acid, converting the enzymebound pyridoxal phosphate to bound pyridoxamine phosphate (2). Other amino acids such as methionine sulfoxide, methionine sulfone, and alanine react much more slowly with the enzyme, but the reaction itself appears t...