Pyrimidine nucleoside monophosphate kinase [deoxycytidine monophosphate:adenosine triphosphate (dCMP:ATP) phosphotransferase. EC 2.7.4.14] has been purified from rat Novikoff ascites hepatoma and rat liver, each to a single major band appearing on sodium dodecyl sulfate polyacrylamide gel electrophoresis. Differences exist in regard to efficiency and regulation of enzymatic activities. The Km v...

Tumor immunity to a transplantable diethylnitrosamine-induced hepatoma in inbred guinea pigs has been found to be immunologically specific and cell mediated. We have investigated this cellular immunity using a quantitative, reproducible, and simple assay based on the ability of leucocytes to inhibit the incorporation of tritiated thymidine (TdR(3)H) by tumor cells. Tumor cell suspensions were o...

A study of the chemical and kinetic properties of a series of transplantable, chemically induced rat hepatomas has revealed profound alterations in isozyme patterns of glycogen synthetase and phosphorylase. Highly differentiated, slow-growing hepatomas retain the liver-type isozymes, but with decreased differentiation and increased growth rate these are largely or entirely replaced by nonhepati...

Nucleolar 28 S, 35 S, and 45 S RNA's were isolated from normal rat liver, Novikoff hepatoma ascites cells, and Morris hepatoma 9618A after short (60 min) and long (6 hr) labeling with orthophosphate-32p. After purification by sucrose density sedimentation, the 32p nucleotide composition of these RNA's was determined. The RNA was hydrolyzed to oligonucleotides with pancreatic ribonuclease. The d...

background: it is now well established that several environmental stress factors cause activation of p38 map kinase and jnk in various cell types to produce chemokines. objective: to investigate the expression of cxc chemokines gro/kc and sdf- 1a in rat's h4 hepatoma cells in response to heat shock, hyperosmolarity and oxidative stress. methods: hepatoma cells were maintained in mem medium...

Bleomycin (BLM) is clinically useful in the treatment of humansquamouscell carcinomas, testicular carcinoma, and malignant lymphoma. The antibiotic is less effective for adenocarcinoma and sarcomas. The less effectiveness of BLMmay be due to lowered BLMsensitivity of tumor cells. The determinant of BLMsensitivity is thought to be drug uptake, BLMhydrolase activity, enzymes involved in DNA break...

1. Twenty minutes after injection of [(3)H]orotic acid into rats the rapidly labelled RNA from the liver is mainly associated with the nuclear fraction and little with the ribosomal cytoplasmic fraction. 2. The thermal denaturation of RNA from the fractions was not as reversible as that of the RNA extracted from whole liver. 3. Rapidly labelled RNA is synthesized by cells from a transplantable ...

Our most recent work [Hibino et al. (1995) Cancer Lett., 88, 49-55] has shown that the selective binding affinities of highly repetitive DNA components for a nuclear scaffold protein from rat ascites hepatoma cells (P230) depend on the degree of sequence-directed bending of the helix axis. In the present experiment, this protein has been highly purified and isolated by a series of column chroma...

1INTRODUCTION Tumor cells have a very distinctive metabolism. Several previous studies have considered the metabolic changes that take place in the host after tumor implantation. Metabolic abnormalities arising from malnutrition and malignancy are seen in animals or patients with cancer cachexia. Various cancers affect the lipid metabolism.1-7) Rats subcutaneously implanted with the ascites hep...

Groups of male weanling rats bearing the transplantable Novikoff ascites hepatoma were fed diets containing graded levels of protein. The food intakes and weight gains were recorded daily. Seven days after inoculation of the rats with the tumor (6 days in Experiment 2), the rats were sacrificed, their organs were weighed, and the tumor and ascites fluid volumes were determined. These results we...