نتایج جستجو برای: as pcr
تعداد نتایج: 5737443 فیلتر نتایج به سال:
Type I trichomes of tomato leaves (Solanum lycopersicum Mill. cv. Moneymaker), as outgrowths the plant epidermis, are suitable for monitoring infection processes powdery mildew species using a high-fidelity digital microscope (DM) without fungal staining. On trichomes, (Pseudoidium neolycopersici L. Kiss) isolate KTP-03 produced maximum four vigorously elongated hyphae per conidium, which stopp...
vocabulary as a major component of language learning has been the object of numerous studies each of which has its own contribution to the field. finding the best way of learning the words deeply and extensively is the common objective of most of those studies. however, one effective way for achieving this goal is somehow neglected in the field. using a variety of activities such as games can r...
this thesis aims at putting together two of the vestest areas of study there is in humanities: bioethics and literature. the two novels in question, oryx and crake and the year of the flood, are considered as some of the most succssful examples of spculative fiction. in these novels the biological and environmental issues raised as looked at from a bioethics point of view.
1Department of Immunology, Waiter Reed Army Institute of Research, Washington, D.C. 20307-5100; 2Tropical Disease Unit, Division of Infectious Diseases, The Toronto Hospital and The University of Toronto, Toronto, Ontario, Canada M5G 2C4 In vitro transcription and translation are powerful tools for examining the s t ructure-funct ion relationships of proteins. Genes can be cloned into plasmid v...
We present a protocol for effi cient amplifi cation of a large number of DNA targets using a single-tube polymerase chain reaction (PCR) based on PCR suppression. This method allows amplifi cation of each DNA target with only one target-specifi c primer whereas the other primer is common for all targets. Thus, this approach requires n+1 primers for n targets instead of 2n in conventional PCR an...
Allele-specific polymerase chain reaction (AS-PCR) is a rapid and cost-effective single nucleotide polymorphism (SNP) genotyping method compared to multiplex or real-time PCR. The SNPs occurring in mitochondrial DNA (mtSNPs) the most abundant humans can be used human identification involving mass fatality incidents. Nevertheless, application of AS-PCR has yet widely applicable forensic investig...
Abstract Objective JAK2 is a non-receptor tyrosine kinase that plays a major role in myeloid disorders. JAK2V617F mutation is characterized by a G to T transverse at nucleotide 1849 in exon 12 of the JAK2 gene, located on the chromosome 9p, leading to a substitution of valine to phenylalanine at amino acid position 617 in the JAK2 protein. Methods In this study we evaluated RNA from 89 pati...
I n the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in vivo, and the study of virus-host relations...
Background and Aim: The JAK2 is an acquired mutation that is observed in majority of patients with classical Philadelphia-negative Myeloproliferative neoplasms that include polycythemia vera (PV), essential thrombocythemia (ET), primary myelofibrosis (PMF). This acquired mutation is characterized by a G to T transversion at nucleotide 1849 in exon 12 of the JAK2 gene, leading to a substitution ...
The respiratory pattern generator modulates the sympathetic outflow, the strength of which is enhanced by challenges produced by hypoxia. This coupling is due to the respiratory-modulated presympathetic neurons in the rostral ventrolateral medulla (RVLM), but the underlining electrophysiological mechanisms remain unclear. For a better understanding of the neural substrates responsible for gener...
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