نتایج جستجو برای: as determined by quantitative elisa and western blot assay

تعداد نتایج: 18291614  

Journal: :Clinical chemistry 2004
Nicolai Grebenchtchikov Arend Brinkman Simone P J van Broekhoven Danielle de Jong Anneke Geurts-Moespot Paul N Span Harry A Peters Henk Portengen John A Foekens C G J Fred Sweep Lambert C J Dorssers

BACKGROUND High concentrations of breast cancer anti-estrogen resistance 1 (BCAR1) protein measured by Western blotting in primary breast tumor cytosols are associated with early disease progression and failure of tamoxifen therapy. The aim of the present study was to develop an ELISA to measure BCAR1 quantitatively in extracts of human breast cancer tissue. METHODS A recombinant fragment of ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده ادبیات و زبانهای خارجی 1393

the current study aimed at investigating the relationship between motivation and test preparation strategies (tpss) used by iranian pre-university students in their preparation period for the university entrance exam (uee). due to the importance of uee in iran, this study also attempted to show its impact on these two important variables. to this end, 100 pre-university students in an iranian p...

پایان نامه :0 1374

what miller wants is a theatre of heightened consciousness. he speaks of two passions in a man, the passion to "feel" and the passion "know". he belives that we can have more of the latter. he says: drama is akin to the other inventions of man in that it ought to help us know more and not merely to spend our feelings. the writing of the crucible shows us that he is trying to give more heightene...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد تهران مرکزی - دانشکده علوم پایه 1390

abstract the cr(iii) and zn(ii) complexes with 2-((9,10-dihydro-1-hydroxy-9,10-dioxaanthracen-4-yloxy)carbonyl)benzoic acid as ligand have been prepared by reacting cr(no3) and zn(no3)2 and ligand in cloroform. the cr and zn complexes with 2-((9,10-dihydro-1-hydroxy-9,10-dioxaanthracen-4-yloxy)carbonyl) were also prepared in chloroform and aceton solutions. the uv-visible and ft-ir spectra of...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد شاهرود - دانشکده پزشکی 1390

nitric oxide (no) is a small molecule synthesized by most of mammalian cells with diverse biological activities including vasodilatation, host defense and wound healing. impaired wound healing is a common occurrence among diabetics and patients receiving glucocorticoid therapy. on the other hand the application of laser in biomedical area have been increased. thus, the current studies were desi...

Journal: :Blood 1998
M J Patel E J Webb T E Shelbourn C Mattia-Goldberg A J George F Zhang E G Moore D J Nelson

Diaspirin cross-linked hemoglobin (DCLHb) is an intramolecularly cross-linked hemoglobin-based oxygen carrier being developed as a therapy for acute blood loss. We report here the absence of immunogenicity of DCLHb in patients enrolled in phase II and III clinical trials of DCLHb. Two very sensitive immunoassays, an enzyme-linked immunosorbent assay (ELISA) and a Western blot assay, were develo...

Gholamreza Abdollahpour Hamid Reza Honarmand, Seyyed Saeed Eshraghi

Background: Leptospirosis is the most common zoonosis widespread in tropical and temperate countries with low social-economic status. We aimed to compare an ELISA kit with an in-house ELISA assay to test the serum samples of the patients who were suspicious of leptospirosis according to their clinical symptoms. Methods: A total of 282 serum samples of patients suspicious of leptospirosis admitt...

Journal: :avicenna journal of medical biochemistry 0
mohammad nadri department of biochemistry, school of medicine, mashhad university of medical sciences, mashhad, ir iran mohammad soukhtanloo department of biochemistry, school of medicine, mashhad university of medical sciences, mashhad, ir iran; cancer molecular pathology research center, ghaem hospital, school of medicine, mashhad university of medical sciences, mashhad, ir iran amir hosein jafarian cancer molecular pathology research center, ghaem hospital, school of medicine, mashhad university of medical sciences, mashhad, ir iran mahboobeh alamdari department of biochemistry, school of medicine, mashhad university of medical sciences, mashhad, ir iran mohsen sisakhti department of biochemistry, school of medicine, mashhad university of medical sciences, mashhad, ir iran tayebeh kianoush department of biochemistry, school of medicine, mashhad university of medical sciences, mashhad, ir iran

results isotype of 1f1 clone was determined to be igg1, which reacted with native protein in the western blot experiment and stained 20% of the membrane of neoplastic cells overexpressing her2 with 3+ grade. however, 3l5 clone showed a low reaction (10%) with native her2 in immunohistochemistry. conclusions the results of both western blotting and immunohistochemistry showed that native her2 ca...

پایان نامه :دانشگاه آزاد اسلامی - دانشگاه آزاد اسلامی واحد تهران مرکزی - دانشکده زبانهای خارجی 1390

acknowledgements i wish to express my gratitude to all those who have helped me in preparing this thesis. i would like to express my deep gratitude to my respected advisor dr. kourosh akef, whose advice and comments helped me in the early stages of the research and throughout the writing process. i would also like to express my gratitude to dr. hajar khanmohammad whose invaluable guidance he...

2010
Xiao-Dan Liu Rui-Fang Fan Yong Zhang Hong-Zhi Yang Zhi-Gang Fang Wei-Bing Guan Dong-Jun Lin Ruo-Zhi Xiao Ren-Wei Huang He-Qing Huang Pei-Qing Liu Jia-Jun Liu

Tanshinone I (Tan-I) is a diterpene quinone extracted from the traditional herbal medicine Salvia miltiorrhiza Bunge. Recently, Tan-I has been reported to have anti-tumor effects. In this study, we investigated the growth inhibition and apoptosis inducing effects of Tan-I on three kinds of monocytic leukemia cells (U937, THP-1 and SHI 1). Cell viability was measured by MTT assay. Cell apoptosis...

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