was precipitated (Kane et al., 1975) with 1,1,3,3-tetramethylurea, extracted with chloroform/methanol (1 : 1, v/v), washed with ether, and dried under N2. Amino acid analysis of this protein corresponded to that of apolipoprotein B, as did its electrophoretic mobility on urea/polyacrylamide-gel electrophoresis. Its specific radioactivity was obtained by radioactivity counting and protein determ...

BACKGROUND AND PURPOSE Apolipoprotein E polymorphism may influence the early development of coronary artery disease. We investigated the putative role of apolipoprotein E phenotypes in cerebral infarction. METHODS The apolipoprotein E phenotypes of 69 patients (mean +/- SD age, 72 +/- 11 years) who had suffered completed stroke or a transient ischemic attack and 68 sex- and age-matched contro...

All classes of lipoproteins considered to be atherogenic contain apo-B100 or apo-B48. However, there is a distinct paucity of data regarding whether lipoproteins containing apo-B48 or apo-B100 differ in their intrinsic ability to promote the development of atherosclerosis. To address this issue, we compared the extent of atherosclerosis in three groups of animals: apo-E-deficient mice (apo-B+/+...

Atherosclerosis is an inflammatory disease of the arterial wall. It is accompanied by an autoimmune response against apolipoprotein B-100, the core protein of low-density lipoprotein, which manifests as CD4 T cell and antibody responses. To assess the role of the autoimmune response in atherosclerosis, the nature of the CD4 T cell response against apolipoprotein B-100 was studied with and witho...

1. The effect of fasting on hepatic mRNA levels of seven plasma proteins was examined in the rat. 2. The levels of mRNA were measured directly in cytoplasmic extracts by hybridization to specific 32P-labelled cDNA probes. 3. Following a 48 h period of fasting, the mRNA levels of apolipoprotein E, apolipoprotein A-IV, albumin, transferrin, and transthyretin decreased by 15-30%, while apolipoprot...

OBJECTIVE This study was carried out to determine whether or not Plasmodium falciparum malaria infection significantly affected apolipoprotein-A1 and cholesterol levels and if apolipoprotein-A1 correlated with the malaria severity in children younger than 5 years old. SUBJECTS AND METHODS Two hundred and fifty-five children, 170 of whom had microscopically confirmed P. falciparum infection, i...

conclusions the findings of this study proved that eight-week interval endurance rope training can have positive effects on lymphocyte abca1 protein expression (as gatekeeper of reverse cholesterol process) and lipid profiles among overweight and obese boy adolescents. objective the current study aimed to investigate abca1 expression in lymphocytes, plasma apolipoprotein a-i and hdl-c in respon...