The health problems caused waterborne pathogens bacteria belonging to the Enterobacteriaceae family are aggravated by rise of antimicrobial resistant phenomenon. This has been identified as one biggest global challenges. present study aims make a comparative approach sensitivity/resistance antibiotics 4 Enterobacter cloacae, Citrobacter freundii, Salmonella typhi and Shigella sonnei isolated fr...

Abstract Background The presence of antibiotic resistant microorganisms in food is great concern globally. This research was carried out to detect and characterize plasmid carriage profiles among members Enterobacteriaceae from different meat types Nigeria. Method From a total 80 samples comprising mutton, pork, beef chicken, organisms belonging the family were isolated by standard procedures i...

Samples from the kidney of 100 diseased fish with signs of haemorrhagic septicemia and 50 samples from outlet water of fish farms were taken aseptically and cultured. In the laboratory, 75 colonies of gram-negative bacteria were biochemically diagnosed as motile aeromonads in our Bacteriology Laboratory Unit using API 20E rapid identification system. The genotype identification using specific p...

In the anterior silk glands (ASGs) of the silkworm, Bombyx mori, intracellular cAMP increases transiently to a very high level shortly after the hemolymph ecdysteroid peak in the prepupal period. In cultured ASGs obtained on the day of gut-purge, cAMP levels were increased by 20-hydroxyecdysone (20E), and this increase was enhanced by an inhibitor of phosphodiesterase, but was not affected by a...

Ecdysis-triggering hormone (ETH) was originally discovered and characterized as a molt termination signal in insects through its regulation of the ecdysis sequence. Here we report that ETH persists in adult Drosophila melanogaster, where it functions as an obligatory allatotropin to promote juvenile hormone (JH) production and reproduction. ETH signaling deficits lead to sharply reduced JH leve...

In addition to the classical nuclear receptor pathway, there is a non-genomic pathway in the cell membrane that regulates gene expression in animal steroid hormone signaling; however, this mechanism is unclear. Here, we report that the insect steroid hormone 20-hydroxyecdysone (20E) regulates calcium influx via phospholipase C gamma 1 (PLCG1) to modulate the protein kinase C (PKC) phosphorylati...


 The aim of this study was ‎the‎ discrimination Salmonella‎‎ isolated from chicken and their feed ‎and drinking water for the epidemiological control salmonellosis. Totally, 289 samples, ‎including 217 cloaca swabs, 46 water, 26 samples were collected five ‎different farms in Karbala governorate, Iraq. Conventional bacteriology tests, API 20E, Vitek 2, serology used bacterial identificati...

Hormone receptor 3 (HR3), an early-late gene of the 20-hydroxyecdysone (20E) signaling pathway, plays a critical role in insect metamorphosis and development. In this study, we identified characterized HR3 (LsHR3) from cigarette beetle, Lasioderma serricorne. The open reading frame LsHR3 is 1581 bp encoding 527 amino acid protein that contains conserved DNA binding domain ligand domain. was mai...

Females of the tussock moth Orgyia recens have vestigial wings, whereas the males have normal wings. During early pupal development, female wings degenerate drastically compared with those of males. To examine whether ecdysteroid is involved in this sex-specific wing development, we cultured pupal wings just after pupation with ecdysteroid (20-hydroxyecdysone, 20E). In the presence of 20E, the ...