Mitochondrial alternative oxidase (AOX) in plants is a non-proton-motive ubiquinol oxidase that is activated by redox mechanisms and 2-oxo acids. A comparative analysis of the AOX isoenzymes AOX1A, AOX1C, and AOX1D from Arabidopsis (Arabidopsis thaliana) revealed that cysteine residues, CysI and CysII, are both involved in 2-oxo acid activation, with AOX1A activity being more increased by 2-oxo...

Complex I is a crucial respiratory enzyme that conserves the energy from NADH oxidation by ubiquinone-10 (Q10) in proton transport across a membrane. Studies of its energy transduction mechanism are hindered by the extreme hydrophobicity of Q10, and they have so far relied on native membranes with many components or on hydrophilic Q10 analogues that partition into membranes and undergo side rea...

The treatment of bleach effluent from pulp and paper Industry for COD, colour and AOX removal was investigated using Ultra violet/Hydrogen peroxide process photocatalysed by Titanium dioxide. The Ultra violet / Titanium dioxide process was able to remove 48% and 37% of COD and colour. The Ultra violet / Hydrogen peroxide process was able to remove 52% and 59% of COD and colour. The combined pro...

Introduction We sought to determine the surgical outcomes of patients with anomalous origin left coronary artery from pulmonary (ALCAPA) enrolled in European Congenital Heart Surgeons Association (ECHSA) database. Materials and Methods From 1999 2019, 907 ALCAPA underwent repair were included current study. The primary outcome was in-hospital mortality. Secondary frequency results concomitant m...

We define the dynamics of spatial and temporal reorganization of the team of proteins and lipids serving peroxisome division. The peroxisome becomes competent for division only after it acquires the complete set of matrix proteins involved in lipid metabolism. Overloading the peroxisome with matrix proteins promotes the relocation of acyl-CoA oxidase (Aox), an enzyme of fatty acid beta-oxidatio...

Cytochrome c oxidase (COX) deficiency is associated with a wide spectrum of clinical conditions, ranging from early onset devastating encephalomyopathy and cardiomyopathy, to neurological diseases in adulthood and in the elderly. No method of compensating successfully for COX deficiency has been reported so far. In vitro, COX-deficient human cells require additional glucose, pyruvate and uridin...

This work evaluated the enzymatic and non-enzymatic antioxidant defense systems of avocados cv. Hass stored at 7 ± 0.5 °C for 10, 20, 30 d, stage edible ripeness. The enzyme system included superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), was composed total phenolic compounds (TPC), carotenoids (TC), α-, β-, γ-tocopherols, as well hydrophilic (H-AOX) lipophilic (L-AOX) activities. ...

Multiple sequence alignments are often used for the identification of key specificity-determining residues within protein families. We present a web server implementation of the Sequence Harmony (SH) method previously introduced. SH accurately detects subfamily specific positions from a multiple alignment by scoring compositional differences between subfamilies, without imposing conservation. T...

Restricted feeding (RF) during daytime is associated with anticipatory activity before feeding, marked hyperphagia after mealtime, adjustments in hepatic metabolism, and the expression of a food-entrained oscillator (FEO). 24 h rhythmicity of liver PPARα, β, and γ, peroxisomal markers (PMP70, AOX, and catalase), and free fatty acids (FFAs) during RF was evaluated. The effect of fasting-refeedin...