BACKGROUND Sand flies are hematophagous arthropods that act as vectors of Leishmania parasites. When hosts are bitten they develop cellular and humoral responses against sand fly saliva. A positive correlation has been observed between the number of bites and antibody levels indicating that anti-saliva antibody response can be used as marker of exposure to sand flies. Little is known about kine...

Neutrophil agglutinins that caused primary autoimmune neutropenia in six young children less than two years of age reported earlier were investigated further for antibody identification and specificity using granulocyte agglutination technique. Initially, the patient sera were tested with parental neutrophils and further confirmed with several donor cells. The sera were tested with autologous n...

Background: Diagnosing typhoid fever definitively, using bone-marrow, blood or feces culture is either invasive, time-consuming, expensive, complicated. Hence, clinicians often use clinical presentations and/or rapid diagnostic methods to examine the indirect evidence of Salmonella typhi infections. This study describes proportion fever, challenges diagnosing and kinetics anti-Salmonella O9 IgM...

Ribonucleoprotein particles containing Sm antigen were separated from particles containing both Sm and RNP antigens by ion-exchange chromatography to study the recognition of these antigens by autoimmune sera. By using the separated antigens, anti-Sm and/or anti-RNP antibodies were detected in approximately 60% of sera from systemic lupus erythematosus patients by both enzyme-linked immunosorbe...

OBJECTIVES Autoantibodies characterizing certain forms of inflammatory myopathy, which are myositis-specific autoantibodies, are useful in the diagnosis and prediction of prognosis in DM/PM. This study aimed to identify a subset of DM patients who have anti-nuclear matrix protein 2 (anti-NXP-2) antibodies by using biotinylated recombinant proteins, and to clarify the clinical features of DM pat...

BACKGROUND The aim of this study was to compare the correlation between new diagnostic methodologies for detecting anti-polymyositis/scleroderma (anti-PM/Scl) IgG antibodies associated with myositis and/or systemic scleroderma assays with existing platforms. METHODS Sera from 164 samples previously tested for anti-PM/Scl IgG antibody by immunodiffusion, ID; 171 sera screened for anti-PM/Scl I...

I N A PREVIOUS PAPER,’ the author reported n a blood sample ( Co) found to be AB, but containing anti-B in the serum. Tile A antigen in tile blood cells was normal when assessed on tile basis of tile reaction of the blood cells ill anti-A sera, whereas tile B cilaracter was weaker than normal and atypical. A study of the family failed to reveal whether tile atypical, weak B-like character was i...

Prostate-specific membrane antigen (PSMA), whose expression is upregulated in poorly differentiated, metastatic, and hormone refractory prostate cancer, could be targeted by gene-based vaccines. The aim of this study was to characterize the humoral immune response against PSMA in prostate carcinoma patients who have been vaccinated against PSMA with gene-based vaccines. Sera from prostate cance...

Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIK fever, which typically manifests as a febrile illness. To develop a CHIKV-specific diagnostic test, CHIKV capsid protein was expressed using a baculovirus expression system. The seroreactvity of the recombinant CHIKV capsid protein was evaluated by ELISA and immuochromatographic assay (ICA), using 40 anti-CHIKV-positive and ...

OBJECTIVES To determine the prevalence and clinical associations of autoantibodies to myeloperoxidase (MPO) in an unselected series of well-characterised outpatients with rheumatoid arthritis (RA) and to compare the distribution of IgG subclasses of anti-MPO antibodies in these patients with that in patients with systemic vasculitis. PATIENTS AND METHODS A study was made of 97 patients with R...