The rat liver sulfate/bicarbonate/oxalate exchanger (sat-1) transports sulfate across the canalicular membrane in exchange for either bicarbonate or oxalate. Sulfate/oxalate exchange has been detected in the proximal tubule of the kidney, where it is probably involved in the reabsorption of filtered sulfate and the secretion of oxalate and may contribute to oxalate-dependent chloride reabsorpti...

The present study examined the overloading of ion-exchange membrane adsorbers, a form of frontal chromatography, as the final purification step in the production of mAbs (monoclonal antibodies) produced from CHO (Chinese-hamster ovary) cells. Preferential binding of impurities over antibody product was exploited using commercially available cation- and anion-exchange membranes. Three different ...

A model system for selective solubilization and fast separation of proteins from the rat liver membrane fraction and purified rat liver plasma membranes for their further proteomic analysis is presented. For selective solubilization, high-pH solutions and a concentrated urea solution, combined with different detergents, are used. After extraction, proteins are separated by anion-exchange chroma...

Alkaline anion–exchange membranes (AAEM) for alkaline polymer electrolyte fuel cells (APEFC) were successfully prepared using electron beam irradiated poly(ethylene–co– tetrafluoroethylene) precursor films grafted with vinylbenzyl chloride (VBC) monomer. The resulting chloromethyl groups were subsequently reacted with trimethylamine to form quaternary ammonium anion–exchange functional head–gro...

The adsorption behavior of anionic dye congo red (CR) from aqueous solutions using an anion exchange membrane (EBTAC) has been investigated at room temperature. The effect of several factors including contact time, membrane dosage, ionic strength and temperature were studied. Kinetic models, namely pseudo-first-order and pseudo-second-order, liquid film diffusion and Elovich models as well as B...

Anion exchanger 1 (AE1) is encoded by SLC4A1 and mediates electroneutral anion exchange across cell membranes. It is the most abundant protein in the red cell membrane, but it is also found in the basolateral membrane of renal alpha-intercalated cells, where it is required for normal urinary acidification. Recently, four point mutations in red cell AE1 have been described that convert the anion...

This work describes the synthesis and characterization of a series poly(2,6-dimethyl-1,4-phenylene oxide) (PPO)-based anion exchange membranes (AEMs), crosslinked with bis-imidazolium cations, investigated as potential ionic conductors for AEM fuel cells. PPO has excellent mechanical thermal properties, its backbone can be functionalized by means several possible routes. Among them, bromination...

In this study, ion exchange nanocomposite membranes was prepared by addition of Mg(OH)2 nanoparticles to a blend containing sulfonated polyphenylene oxide and sulfonated polyvinylchloride via a simple casting method. Magnesium hydroxide nanoparticles were synthesized via a facile sono-chemical reaction and were selected as filler additive in fabrication of ion exchange nanocomposite membranes. ...