Intensive studies have been carried out on the rotational spectra of various isotopic species of ketene, H2C = C = 0 , since 1950 [ 1 6 ] . The centrifugal distortion analysis of ketene [3, 4 ] , together with information from additional infrared data [3, 5 ] , enabled Mallinson and Nemes [6] to reevaluate the molecular force field for ketene. Recently, microwave investigations on the higher ho...

Even for moderately sized proteins, the multiple occurrence of cysteine and lysine residues often prevents the specific labeling of polypeptides with a single probe. To increase specificity, a method was developed to convert the commonly available succinimidyl esters of fluorescent dyes into benzyl thioesters via trimethyl aluminum-activated benzyl mercaptan. The thioester can then be reacted v...

The amounts of volatile substances responsible for the malodor of human waste (feces and urine) obtained from the storage tank of a community waste-water treatment plant were determined. Thus far, there has been little systematic research on malodor-causing substances of human waste. These substances were collected using Tenax-TA, and their concentrations were determined by the usual thermal-de...

Methyl mercaptan is a naturally occurring compound known to be formed and utilized by living organisms. It can arise in microorganisms (1) and in the rat (2) from methionine. It is rapidly oxidized in the rat, and its methyl carbon atom is incorporated into a number of compounds in this animal (3). In yeast cultures it promotes the accumulation of thiomethyl adenosine (4). Reported here is a re...

Acetaldehyde has recently been added to the list of chemicals in the control law for offensive odor in Japan, and its concentration in the atmosphere is limited by the law to the range of 0.05 to 0.5ppm1). Acetaldehyde is emitted into the atmosphere particularly from the factories producing acetic acid, vinyl acetate, and chloroprene. It is well known that acetaldehyde irritates the mucous memb...

The first example of a biocatalytic strategy for the synthesis of thioethers via an intermolecular carbene S-H insertion reaction is reported. Engineered variants of sperm whale myoglobin were found to efficiently catalyze this C-S bond forming transformation across a diverse set of aryl and alkyl mercaptan substrates and α-diazoester carbene donors, providing high conversions (60-99%) and high...