Digital PCR has become the emerging technique for the sequence-specific detection and quantification of nucleic acids for various applications. During the past years, numerous reports on the development of new digital PCR methods have been published. Maturation of these developments into reliable analytical methods suitable for diagnostic or other routine testing purposes requires their validat...

The concept of a plane scatterer that was developed earlier for scalar waves is generalized so that polarization of light is included. Starting from a Lippmann-Schwinger formalism for vector waves, we show that the Green function has to be regularized before T matrices can be defined in a consistent way. After the regularization, optical modes and Green functions are determined exactly for fini...

BACKGROUND Measurement of inulin clearance is considered to be the gold standard for determining kidney function in children, but this method is time consuming and expensive. The glomerular filtration rate (GFR) is on the other hand easier to calculate by using various creatinine- and/or cystatin C (Cys C)-based formulas. However, for the determination of serum creatinine (Scr) and Cys C, diffe...

Considerable progress has been made on the development of a variety of analytical methods to aid in the carcinogenic risk associated with exposure to both occupational and environmental agents. Although the development of these methods has been accompanied by consideration of many statistical issues, there are many areas where additional effort could be directed if these analytical methods are ...

in this study, he’s variational approach (va) is used to solve the non-naturalvibrations and oscillations. the method works very well for the whole range of initial amplitudesand does not demand small perturbation. it is also sufficiently accurate to both linear and nonlinearphysics and engineering problems. we consider some examples to illustrate the effectiveness andconvenience of the method....

methods allplex gi-virus assay was developed to detect pathogens causing viral gastroenteritis, one of the major diseases caused by rna viruses. this one-step multiplex real-time polymerase chain reaction (pcr) based on the mudt technique permits simultaneous amplification and detection of target nucleic acids of norovirus gi, norovirus gii, rotavirus a, adenovirus f, astrovirus, and sapovirus ...

A Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method was developed and validated for theestimation of Sofosbuvir which is a new antiviral drug. The RP-HPLC succeeded on Phenomenex Luna® LC C18column (150×4.6mm, 5μm). mobile phase effective according to the polarity studied phasewas consisting Acetonitrile: Methanol: Water in ratio 50:30:20 v/v/v using at flow rate 1ml/min. w...

Since the adoption of the ICH Q8 document concerning the development of pharmaceutical processes following a quality by design (QbD) approach, there have been many discussions on the opportunity for analytical procedure developments to follow a similar approach. While development and optimization of analytical procedure following QbD principles have been largely discussed and described, the pla...