Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities potential amylases, however, cannot be quantified directly from supernatant due to high background activity native α-amylase. This caused by gene products amyA, amyB, and amyC. In this study, in vitro CRISPR/Cas9 system was established A. delete these...

Cereal Chern. 68(4):383-390 Amylases from germinating maize (cv. B73) were fractionated by affinity chromatography, anion exchange chromatography, and chromatofocusing. Two groups of amylase enzymes were separated by affinity chromatography. About one half of the total amylase activity was bound on a cycloheptaamylose-Sepharose 6B column. Bound proteins were fractionated by anion exchange into ...

BOA (2-benzoxazolinone) is a potent phytotoxin present in several graminaceous crops such as rye, maize and wheat. Due to its wide range of phytotoxicity, it is considered as a potential pesticide. A study was conducted to explore the impact of BOA on the radicle and plumule elongation of mung bean (Phaseolus aureus) and associated changes in the macromolecular content - proteins and carbohydra...

Carbohydrases find a wide application in industrial processes and products, mainly in the food industry. With these enzymes, it is possible to obtain different types of sugar syrups (viz. glucose, fructose and inverted sugar syrups), prebiotics (viz. galactooligossacharides and fructooligossacharides) and isomaltulose, which is an interesting sweetener substitute for sucrose to improve the se...