We compared two methods to generate polymorphic markers to investigate the population genetics of Trypanosoma evansi; random amplified polymorphic DNA (RAPD) and amplified restriction fragment length polymorphism (AFLP) analyses. AFLP accessed many more polymorphisms than RAPD. Cluster analysis of the AFLP data showed that 12 T.evansi isolates were very similar ('type A') whereas 2 isolates dif...

Gene expression analyses using spotted cDNA microarrays typically require relatively large quantities of total RNA (up to 100 microg) or polyA+RNA (1-5 microg). However, samples obtained by microdissection, patient biopsies, or embryonic samples often are small and yield an insufficient amount of RNA. Methods such as linear RNA amplification by in vitro transcription (IVT) or cDNA amplification...

background and objective:determination of her2 gene is crucial in breast carcinoma management and prognosis, as her2 alterations are linked to a shorter disease-free period, overall survival and resistance to tamoxifen anti-estrogen therapy and other chemotherapy regimens, regardless of the nodal or hormone receptor status. this study aimed to estimate her2 gene status of infiltrative mammary c...

In this study, we used Random Amplified Polymorphic DNA (RAPD) for identification of 17 isolates of Leishmania from the skin and reticuloendothelial system of humans, animal reservoirs (rodent and dog) and sandflies in various parts of Iran in the last decade. Fifteen species have been confirmed by isoenzyme characterization by the London School of Hygiene and Tropical Medicine and Shiraz ...

The scattering of test fields by sandwich waves has been studied extensively. It found that for a variety fields, the energy scattered is amplified. In this paper, Scattering gravitational are calculated solving perturbations in wave background. Dependence their amplification with respect to parameters and incident examined. results show some cases, outgoing amplified as well.

Random Amplified Polymorphic DNA (RAPD) analysis of Pistacia vera L. was used to distinguishing sex type in progeny plants. Varied genotypes of the pistachio were analyzed by PCR amplification of a ten base primer previously found to be linked to the expression of a 945bp amplification band in female trees and its absence in males. Results revealed that genetic material from cultivar samples am...

The human TAX-1 gene encodes a Mr 135,000 glycoprotein that is transiently expressed on the surface of a subset of neurons during development and is involved in neurite outgrowth. The TAX-1 gene has been mapped to a region on chromosome 1 that has been implicated in microcephaly and the Van der Woude syndrome. Using restriction landmark genome scanning to search for amplified genes in gliomas, ...

SHACAL is a 160-bit block cipher based on the hash standard SHA-1, as a submission to NESSIE. SHACAL uses the XOR, modular addition operation and the functions of bit-by-bit manner. These operations and functions make the differential cryptanalysis difficult, i.e, it is hard to find a long differential characteristic with high probability. But, we can find short differential characteristics wit...

We present two configurations of an amplified fiber-optic-based corrosion sensor using the optical time domain reflectometry (OTDR) technique as the interrogation method. The sensor system is multipoint, self-referenced, has no moving parts and can measure the corrosion rate several kilometers away from the OTDR equipment. The first OTDR monitoring system employs a remotely pumped in-line EDFA ...

Jan Radil, CESNET, a.l.e. , Zikova 4, 160 00 Prague 6, Czech Republic, [email protected] Leoš Bohác, Czech Technical University in Prague, Technická 2, 166 27 Prague 6, Czech Republic, [email protected], also with CESNET, a.l.e. Miroslav Karásek, Institute of Radio Engineering and Electronics, Academy of Science of Czech Republic, Chaberská 57, 182 51 Prague 8, Czech Republic, [email protected]...