Obtaining full-length or even near full-length cDNA clones has been a time-consuming and labor-intensive step in the analysis of cloned genes. Recent methods which use PCR as a preparative tool for cloning (1,2) have made this step considerably more rapid, but may introduce sequence errors in the resulting clones due to numerous sequential rounds of in vitro replication. We describe a method fo...

The PETSc (Portable Extensible Tools for Scientiic computing) library arose from research into domain decomposition methods which require combining many diierent solutions in a single application. The initial eeorts tried to use existing numerical software but had limited success. The problems include everything from faulty assumptions about the computing environment (e.g., how many processes t...

Assembly of DNA 'parts' to create larger constructs is an essential enabling technique for bioengineering and synthetic biology. Here we describe a simple method, PaperClip, which allows flexible assembly of multiple DNA parts from currently existing libraries cloned in any vector. No restriction enzymes, mutagenesis of internal restriction sites, or reamplification to add end homology are requ...

Digital libraries are becoming more ubiquitous and their contents can be of interest not only to the communities that create them but also to individuals and organizations around the world. The content of most digital libraries is not visible to web search engines and the internal search engines of many digital libraries do not support cross language access. In this paper, we present some ideas...

During the fall of 2015, the Augusta University Libraries began the process of implementing Ex Libris’ nextgeneration library management solution, Alma. This process is occurring in various phases, with the initial steps being data clean-up and migration. As part of the migration process, cataloging records that are currently created and maintained by both university libraries will be migrated ...

introduction: this is a descriptive study to study the conditions of hospital libraries at iran ,tehran and shahid beheshti university of medical sciences and compares the staff, printing sources, services and physical situations with “standards for hospital libraries 2002” and “standard of educational and treatment center library collection”. methods: this survey has been done on 35 hospital l...