Aim. To analyse effectiveness of introduced genes ornithine-δ-aminotransferase (oat) Medicago truncatula and fragments two copies first exon intron the gene prolinedehydrogenase (pdh) Arabidopsis thaliana, that form double stranded RNA suppressor wheat, in increase her productivity for actions ofosmotic stress. Methods. Determination content free L-proline (Pro); activity enzyme (OAT), proline ...

It is well known that GABA plays an inhibitory role in crustacean ans mammalian central nervous system [ 1,2]. Since GABA does not cross the blood-brain barrier, various inhibitors of GABA aminotransferase which cause an increase in its cerebral level have been introduced into this field of research [3-121. Most of these inhibitors are very toxic and not specific for GABA aminotransferase. Duri...

The parameters of protein metabolism, such as the levels of total, soluble proteins and free amino acids and also the activity levels of protease, glutamate dehydrogenase, aspartate aminotransferase and alanine aminotransferase were assayed in the silk gland of the fifth instar larva of silkworm, Bombyx mori, under the impact of ultrasound. In general ultrasound has an elevatory effect on these...

The genus Phyllanthus (family Euphorbiaceae) is considered one of the important medicinal and ornamental plants. A phytochemical analysis of the extracts was performed to search for the active ingredient. Results of the investigation of the hepatoprotective activity of Phyllanthus atropurpureus Boj. Hort. Maurit. revealed that the activities of alcoholic extracts of its aerial parts and roots w...

Dietary habits are often considered as a pathogenic factor for fatty liver. The impact of dietary intake and steatosis on drug metabolism remains poorly investigated. Our aim was to assess the effect of dietary intake on in vivo cytochrome P450 (CYP) activities in eleven patients with abnormal liver function tests potentially due to fatty liver and associated with a high-sugar diet. Liver funct...

1. A method was devised to allow determination of intramitochondrial aspartate amino-transferase activity in suspensions of intact mitochondria. 2. Addition of purified rat liver mitochondrial aspartate aminotransferase to suspensions of rat liver mitochondria caused an apparent increase in the intramitochondrial enzyme activity. No increase was observed when the mitochondria were preincubated ...

To evaluate the protective activity of fruits against liver injury, 22 different fruits were fed to rats with liver damage caused by D-galactosamine, a powerful liver toxin. As measured by changes in the levels of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST), avocado showed extraordinarily potent liver injury suppressing activity. Five active compounds were isolate...

The catalytic activity concentrations of aspartate aminotransferase (EC 2.6.1.1) and alanine aminotransferase (EC 2.6.1.2) in several commercial control sera were determined at three different temperatures. Although these sera were of various biological origins, the temperature-conversion factors calculated are nearly identical to those for native human sera. The proportional increase of the ac...

The studies reported here support the observation that elevated excretion of ~-hydrox~henylp~~uvic acid could occur in the presence of deficient hepatic tyrosine aminotransferase activity. The ketoacid need not have come either from the liver or from the kidney. It appears possible that the urinary ketoacid, both in tyrosine aminotransferase deficiency and in phenylketonuria, originates not in ...