Abstract Recent studies have revealed the prevalence and biological significance of guanidine metabolism in nature. However, metabolic pathways used by microbes to degrade or mitigate its toxicity not been widely studied. Here, via comparative proteomics subsequent experimental validation, we demonstrate that Sll1077, previously annotated as an agmatinase enzyme model cyanobacterium Synechocyst...

Human interleukin-3 (IL-3) is a hematopoietic growth factor involved in the survival, proliferation and differentiation of multipotent cells. However, recombinant IL-3 usually expressed as insoluble form (inclusion bodies) Escherichia coli This state protein often shows no bioactivity. Herein, we report simple method for solubilization, refolding purification human E. First, was JM109 (DE3) aft...

We describe a new class of potent, non-amide-based small molecule thrombin inhibitors in which an amidinohydrazone is used as a guanidine bioisostere on a non-peptide scaffold. Compound 4 exhibits nM inhibition of thrombin, is selective for thrombin, and shows 60 and 23% bioavailability in rabbits and dogs, respectively. Crystallographic analysis of 4 bound to thrombin confirmed the amindinohyd...

New wedge-shaped thermotropic liquid crystalline materials containing a guanidinium moiety at the apex organize into various supramolecular structures such as hexagonal columnar, rectangular columnar and Pm3n cubic mesophases depending on anions illustrating guest-directed self-organization in mesophases.

In the title compound, C(18)H(29)N(3)O, a polysubstituted guanidine, the torsion angles indicate that the guanidine unit and the carbonyl group are almost perpendicular to one another [O-C-N-C= -7.40 (18), C-N-C-N= -97.21 (15) and 86.41 (13)°]. The crystal packing is stablized by inter-molecular N-H⋯O hydrogen bonds, which link the mol-ecules into a chain.

Paramagnetic relaxation has been used to monitor the formation of structure in the folding peptide chain of guanidinium chloride-denatured acyl-coenzyme A-binding protein. The spin label (1-oxyl-2,2,5,5-tetramethyl-3-pyrroline-3-methyl)methanesulfonate (MTSL) was covalently bound to a single cysteine residue introduced into five different positions in the amino acid sequence. It was shown that ...

ment, together with a summary of the remarkable chemical reactivity of this unit. Ion exchange chromatography (Dowex 50Wx8) of the acid hydrolysate (10N hydrochloric acid, reflux for 24 hours) of viomycin afforded two basic amino acids, viomycidine and a small amount of a compound which we have termed viocidic acid. Viomycidine was characterised as its crystalline monohydrochloride m. p. 200~20...

Transport properties of membrane vesicles isolated from two adenosine triphosphatase-deficient mutants of Escherichia coli, NR70 and DL54, were compared with those of vesicles prepared from the corresponding parental strains. As reported previously (Rosen, 1973; Altendorf et al., 1974), vesicles prepared from these mutants grown under aerobic conditions exhibited defective amino acid transport,...