ß-glucuronidase gene (GUS) driven by a constitutive 35S CaMV-promoter in Scots pine (Pinus sylvestris L.) buds were studied by the microprojectile DNA-delivery method. Buds were collected from 5-, 15and 50-year-old trees. In buds from all age groups the amount of transient expression was dependent on the season; the highest values were found in March, and values were lowest both at the beginnin...

By definition, a dormant vegetative bud is viable but it fails to grow when subjected to environmental conditions normally favorable for growth. An understanding of the biochemistry of dormant and active buds is needed to serve as a basis for solving the problem of artificially breaking or inducing bud dormancy. The present study was conducted with the aim of adding to existing knowledge concer...

A male flower-specific gene SlMF1 was isolated from male flower buds of the dioecious plant Silene latifolia. SlMF1 is expressed in all the floral meristems at the very early stage of development in both male and female flower buds. At the mature stage of development in male flower buds, SlMF1 transcripts were specifically accumulated in pollen mother cells, tapetal cells, and the developing ti...

Dioxygenase, Flavonoid Biosynthesis, Flavonols, Matthiola incana Soluble enzyme preparations from flower buds of Matthiola incana catalysed the conversion of dihydrokaempferol to kaempferol and of dihydroquercetin to quercetin. The reaction required 2-oxoglutarate, ascorbate and Fe2+ as cofactors and had a pH-optimum at about 7.0. Highest enzyme activity was already present in the youngest buds...

In high-latitude regions, the cold hardiness of buds and canes grapevine is important for budburst time yield in next season. The freezing resistance sampled from six wine grapes currently cultivated Hokkaido, Japan, all them grown autumn to winter, was investigated. A significant difference between cultivars their detected harvested winter. addition, outstanding differences lower temperature e...

Abstract Some obstacles are associated with in vitro propagation of date palm, such as explant tissue browning, slow callus growth and development, low organogenesis multiplication efficiency, frequent vitrification. This investigation studied the effect five types gelling agents (Danish Agar, Cero Agar Type 8952, Chile Gerlite Food Grade, Agar-Agar.) on regeneration bud Phoenix dactylifera L. ...

delaying bud break, using chemicals is an approach to avoid spring frost damage. therefore, the effect of spraying different concentrations of soybean oil and naphthalene acetic acid (naa) on delaying bud break, altering internal atmosphere and cold hardiness in grape cultivar ‘fakhri’ was studied in 2011 in a commercial vineyard in maryanaj, hamedan using a factorial trial based on randomized ...