trypsin is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. trypsin has been used widely in various biotechnological processes. in this study trypsin was purified using two precipitation cut, 35% and 75% as saturation percent of ammonium sulfate solutions, tca precipitation (2.5% w/v), ph precipitation and finally affinity chromatography. this m...

Two experiments were conducted to investigate the effects of sodium sulfate water and the efficacy of nonnutritive feed additives in nursery pig diets. In Exp. 1, 320 barrows (5.4 ± 0.1 kg BW and 21 d of age) were allotted to 1 of 8 treatments for 24 d in a 2 × 4 factorial with 2 levels of sodium sulfate water (control or 3,000 mg sodium sulfate/L added), and 4 dietary zeolite (clinoptilolite) ...

groundwater is a major source of water for domestic, industrial, and agricultural sectors in many countries. the main objective of this research was to provide an overview of present groundwater quality using parameters such as calcium, magnesium, sodium, chloride, sulfate, ph, and electrical conductivity (ec) in the mehran plain, ilam province using gis and geostatistical techniques. a total o...

Tetraethoxysilane (TEOS, 28%), n-cetyltrimethylammonium bromide (CTAB, ≥99%), ammonium hydroxide solution (25%), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 99%), N-Hydroxysuccinimide (NHS, 98%), 3-Aminopropyltriethoxy-silane (APTES, 99%), succinic anhydride (99%), sodium citrate tribasic dihydrate, tannic acid, and Rhodamine B were purchased from Sigma Company. Hydrogen tetrachloroaur...

OBJECTIVES Decellularization of aortic valve allografts in advance of transplantation is a promising approach to overcome immune-induced early graft failure. In this study the effects of in vitro cell extraction on extracellular matrix molecules and in vivo remodeling of decellularized aortic valves were investigated in a heterotopic aortic valve rat implantation model. METHODS Rat aortic val...

the most important environmental pollutant in steel industry is air pollution due to the process of its products. optimise sitting for this industry, in a great extend will prevent pollutants and emissions. khorasan steel company is located near some populous villages and three rivers. it is necessary to perform an investigation for providing abatement and control of air pollution, in time of p...

The Development of an analytical method for the determination of 24 multiclass pesticides in mango at the ≤10 ng g level. The method involves extraction of 10 g of homogenized mango samples (2 g of Sodium chloride+8g of Magnesium sulfate) with 10 mL of Acetonitrile; Clean up by Dispersive solid phase extraction with a combination of primary secondary amine (PSA), graphitized carbon black (GCB) ...