As part of the development of enzyme-mediated cancer imaging and therapy, a novel technology to entrap water-insoluble radioactive molecules within solid tumors, we show that a water-soluble, radioactive quinazolinone prodrug, ammonium 2-(2'-phosphoryloxyphenyl)-6-[125I]iodo-4-(3H)-quinazolinone (125IQ(2-P)), is hydrolyzed by alkaline phosphatase to a water-insoluble, radiolabeled drug, 2-(2'-h...

Chemical recycling of textiles holds the potential to yield materials equal quality and value as products from virgin feedstock. Selective depolymerization textile polyester (PET) regenerated cellulose/PET blends, by means alkaline hydrolysis, renders monomers PET while cellulose remains in fiber form. Here, we present mechanism reactivity during hydrolysis. Part I this article series focuses o...

Alkaline phosphatase has been purified from microsomes of chicken epiphyseal cartilage by first selectively extracting certain adventitious proteins with 0.25 M trichloroacetate. The membrane-bound enzyme was then solubilized by 1% cholate in buffered 33% saturated ammonium sulfate and purified by column chromatography on Bio-Gel A-5m, extraction with 1-butanol, and ion exchange chromatography ...

Neutral lipolytic activity (hydrolysis of olive oil at 37”, pH 7.0) and alkaline lipolytic activity (hydrolysis of tributyrin at 47’, pH 8.0) have previously been demonstrated in human adipose tissue. Neutral lipolytic activity has currently been shown to be present in a large molecular weight substance, associated with lipid, and separable from most alkaline lipolytic activity by ammonium sulf...

0022-2860/$ see front matter Published by Elsevier doi:10.1016/j.molstruc.2010.01.048 q Mention of trade names or commercial products purpose of providing specific information and does n endorsement by the US Department of Agriculture. * Tel.: +1 215 233 6680; fax: +1 215 233 6795. E-mail address: [email protected] Investigations of keratins extracted from wool by reduction hydrolysis ...

1. Particulate enzyme preparations obtained from Bacillus stearothermophilus B65 by digestion with lysozyme were shown to catalyse teichoic acid synthesis. With CDP-glycerol as sole substrate the preparations synthesized 1,3-poly(glycerol phosphate). It was characterized by alkaline hydrolysis, by glucosylation to the alkali-stable 2-glucosyl-1,3-poly(glycerol phosphate) with excess of UDP-gluc...

The environmental impacts of energetic compounds can be minimized through the design and selection of new energetic materials with favorable fate properties. Building predictive models to inform this process, however, is difficult because of uncertainties and complexities in some major fate-determining transformation reactions such as the alkaline hydrolysis of energetic nitroaromatic compounds...

The investigation of the structure of the lecithins and cephalins (phosphatidylethanolamines) from natural sources gave rise to the question, whether the phosphoric acid in these substances is attached to the (Yor P-carbon atom of the glycerol moiety. Since the acid and alkaline hydrolysis of natural lecithins yielded mixtures of CYand p-glycerophosphoric acid (GPA), it was assumed that they ex...

The increasing amount of seaweed accumulated in the coasts calls for a proper disposal or treatment order to reduce secondary pollution effects. Anaerobic digestion is technology that simultaneously produces biogas, supporting further expansion renewable energy production through sustainable bioresources. In this study, three pretreatments (alkaline, hydrothermal and thermo-acidic) were applied...