OBJECTIVE Collagen peptides (CP) compounds, as bone health supplements, are known to play a role in the treatment of osteoporosis. However, the molecular mechanisms of this process remain unclear. This study aimed to investigate the effects of bovine CP compounds on the proliferation and differentiation of MC3T3-E1 cells. METHODS Mouse pre-osteoblast cell line MC3T3-E1 subclone 4 cells were t...

BACKGROUND Abdominal aortic aneurysm (AAA) is a life-threatening disease and its prevalence rate increases with social aging. The degradation of elastic is an important factor in the formation of AAA. METHODS Adipose derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (BMSCs) were isolated from rats, and identified by Oil red O and alizarin red staining after adipogenesis and os...

The article concerns with the prospects of using phenolphthalein test solution in practice surveying concrete and reinforced building structures planta. problem study is limits application on pH level determined (the indicator works only at 8≤rn≤10). There a need expanding application. In order to determine different zones we have modernize method by adding other indicators phenolphthalein. sta...

A new chelating resin was prepared by coupling Amberlite XAD-4 with alizarin red-s through an azo spacer, characterized by infra-red spectroscopy and thermal analysis and studied for Rh(III) preconcentration using inductively coupled plasma atomic emission spectroscopy (ICP-AES) for rhodium monitoring in the environment. The optimum pH for sorption of the metal ion was 6.5. The sorption capacit...

METHODS: Human umbilical cords were harvested following routine delivery and WJ was isolated and purified. In vitro, WJ derived stem cells were placed in osteogenic differentiation medium for 14 days, followed by Alizarin Red S staining to evaluate mineral deposition. In vivo, we used a rat critical-size alveolar bone defect model to investigate the use of Wharton’s Jelly (WJ) in formation of b...

The role of fibroblast growth factor 9 (FGF9) in bone formation may depend on gene dosage, developmental stage, cell type or interactions with other cytokines. In the present study bone marrow stromal stem cells (BMSCs) and dental pulp stem cells (DPSCs) were cultured and osteogenically induced in vitro, treated with exogenous FGF9 at varying concentrations. Alkaline phosphatase staining, aliza...

The kidneys of a 3-month-old female cat were examined. The cat which had been raised under controlled conditions with no history of any poisoning showed progressive weight loss with increases in blood BUN and creatinine concentrations. At necropsy, both kidneys were firm in consistency with formation of focal scars. Histopathologically, widespread deposition of crystals was observed in the rena...

OBJECTIVES Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage. MATERIALS AND METHODS Isolated rBMMSc were grown in DMEM supplemented with 10% FBS. The cells were exposed to different concentra...