No. Alla4302 Beamline(s): X8C Introduction: 2-Keto-3-deoxy-6-phosphogluconate (KDPG) aldolase catalyses the reversible cleavage of KDPG to pyruvate and glyceraldehyde-3-phosphate. The enzyme is a class I aldolase whose reaction mechanism involves formation of Schiff base intermediates between Lys-133 and a keto substrate. A covalent adduct was trapped by flash freezing 2-keto-3-deoxy-6-phosphog...

Background: Statins frequently cause myopathy especially in combination with fibrates, and physical activity is considered a trigger for the muscle disorder. Elevated plasma levels of creatine kinase (CK), lactate dehydrogenase (LDH) and aldolase, are the main indicators of the severity of myopathy. Carvedilol is commonly used with lipid-lowering drugs in the management of heart failure, hypert...

Böck, August (Purdue University, Lafayette, Ind.), and Frederick C. Neidhardt. Properties of a mutant of Escherichia coli with a temperature-sensitive fructose-1,6-diphosphate aldolase. J. Bacteriol. 92:470-476. 1966.-A mutant of Escherichia coli in which fructose-1,6-diphosphate aldolase functions at 30 C but not at 40 C was used to study the physiological effect of a specific block in the Emb...

Giardia lamblia fructose-1,6-bisphosphate aldolase (FBPA) is a member of the class II zinc-dependent aldolase family that catalyzes the cleavage of d-fructose 1,6-bisphosphate (FBP) into dihydroxyacetone phosphate (DHAP) and d-glyceraldehyde 3-phosphate (G3P). In addition to the active site zinc, the catalytic apparatus of FBPA employs an aspartic acid, Asp83 in the G. lamblia enzyme, which whe...

Aldolase catalyzes the reversible cleavage of 1 mole of fructose-l ,6diphosphate (HDP) into 1 mole of glyceraldehyde-3-phosphate and 1 mole of dihydroxyacetone phosphate (1, 2). Although the enzyme has been obtained in crystalline form by Warburg and Christian (3) and by Taylor, Green, and Cori (4) only fragmentary data concerning the content of aldolase in different mammalian tissues have been...

The amino acid residues in the carboxyl-terminal region of fructose diphosphate aldolase isolated from fresh rabbit liver are -(Gly,Phe,Leu,Ala,Thr2,Ser2)-Tyr. This differs from the carboxyl-terminal structure in aldolase isolated from tissue which had been frozen and thawed. Amino acid analyses, ultracentrifugation, and digestion with carboxypeptidase suggest that the difference is the result ...

Aldolase catalyzes the reversible cleavage of 1 mole of fructose-l ,6diphosphate (HDP) into 1 mole of glyceraldehyde-3-phosphate and 1 mole of dihydroxyacetone phosphate (1, 2). Although the enzyme has been obtained in crystalline form by Warburg and Christian (3) and by Taylor, Green, and Cori (4) only fragmentary data concerning the content of aldolase in different mammalian tissues have been...