Three turbidometric methods and one method using ultraviolet spectrophotometry for estimating total cerebrospinal fluid protein have been examined. The necessity for preliminary dialysis renders the ultraviolet method unsuitable for routine use. The turbidometric method of Meulemans (1960) using a sulphosalicylic acid-sodium sulphate precipitating fluid is better than a method using sulphosalic...

On hyperimmunization of rabbits with pneumococcal vaccine, increase in the content of gamma globulin and fall of albumin in the serum are produced. In such hyperimmunized rabbits, investigations of the plasma volume, albumin degradation, and the distribution of the albumin intra- and extravascularly were undertaken by Sterling's method employing rabbit albumin labelled with I(131). The investig...

1. Highly purified rabbit Type III pneumococcus anticarbohydrate proved to be homogeneous in the ultracentrifuge and its sedimentation constant, 7.0.10(-13), did not differ from that of the principal component of normal rabbit globulin or of immune rabbit globulin containing up to 50 per cent of anti-egg albumin. The molecular weight of antibody in the rabbit is therefore probably very close to...

To further evaluate thyroid function in patients with liver disease, we have measured total and free T3 and T4, thyroxine binding globulin, basal and thyrotropin releasing hormone-stimulated thyrotropin and thyroglobulin antibodies in 33 patients with liver cirrhosis, in 22 with chronic hepatitis and in 30 healthy controls. All the patients but one were clinically euthyroid. T3, FT3, T3/thyroxi...

It is necessary to assess the state of nitrogen metabolism deepen control over usefulness feeding cattle and ensure prompt response nutritional imbalances diet adjustments. Nitrogen balance a key indicator metabolism. The authors presented results determining content variability indicators in Holstein Western Siberia. object study was bulls breed, obtained from four sires conditions industrial ...

Sequential IgE-binding epitopes were identified on the molecular surface of Pis v 1 (2S albumin), 2 (11S globulin/legumin) and 3 (7S globulin/vicilin)—major allergens from pistachio (Pistacia vera) seeds—using Spot technique. They essentially consist hydrophilic electropositively charged residues well exposed allergens. Most epitopic regions do not coincide with putative N-glycosylation sites t...

Normal rats subjected to end-to-side portacaval shunt showed decreased survival and weight gain, a progressive fall in serum albumin and reciprocal rise in serum gamma globulin when compared with sham-operated controls for 12 weeks. Antibacterial lipopolysaccharide antibody was detected in significant titre at the sixth and twelfth weeks. It is suggested that the elevated levels of gamma globul...

Rats bearing Morris liepatoma 7800 and Morris hepatoma 7777 have serum protein compositions which differ from normal rats. There is a slight decrease of albumin and an increase in a-globulin proteins in the circulating blood of the tumor-bearing animals. The increase in a-globulin is larger than would be expected if globulin would just compensate for the slight decrease in albumin, resulting in...

Ninety-six patients were observed at intervals for as long as three and one-half years in some cases. In most instances multiple determinations were made during this time using the electrophoretic pattern of the proteins before and after cold ether extraction (McFarlane method), to measure lipid transport. Hyperlipemia usually produced an increased in beta globulin. A varying decrease in albumi...