نتایج جستجو برای: agrobacterium

تعداد نتایج: 5603  

2009
Monica T. Britton Matthew A. Escobar Abhaya M. Dandekar

The common soil bacteria Agrobacterium tumefaciens and Agrobacterium rhizogenes are unique genetic pathogens capable of fundamentally redirecting plant metabolism in order to generate macroscopic tissue masses (crown galls and hairy roots, respectively) which support the growth of large populations of Agrobacteria. Central to pathogenesis is the horizontal transfer of a suite of oncogenes from ...

2012
JIUN Y. YEN KATHERINE M. BROADWAY

31 The flagellotropic phage 7-7-1 specifically adsorbs to Agrobacterium sp. H13-3 (formerly 32 Rhizobium lupini H13-3) flagella for efficient host infection. The Agrobacterium sp. H13-3 33 flagellum is complex and consists of three flagellin proteins, the primary flagellin FlaA which is 34 essential for motility and the secondary flagellins FlaB and FlaD which have minor functions in 35 motilit...

2014
Evans Nyaboga Jaindra N. Tripathi Rajesh Manoharan Leena Tripathi

Although genetic transformation of clonally propagated crops has been widely studied as a tool for crop improvement and as a vital part of the development of functional genomics resources, there has been no report of any existing Agrobacterium-mediated transformation of yam (Dioscorea spp.) with evidence of stable integration of T-DNA. Yam is an important crop in the tropics and subtropics prov...

2013
Y. M. Moiseeva V. A. Velikov I. V. Volokhina Yu. S. Gusev O. S. Yakovleva M. I. Chumakov

Aims: The aim of this study was to estimate the efficiency of T-DNA transfer during Agrobacterium-mediated transformation of maize (Zea mays L.) at different temperatures. In addition, the way of T-DNA transfer was studied after application of an Agrobacterium suspension at maize pistil filaments. Study Design: Transgenic maize plants were obtained with an antisense suppressor of the proline de...

2002
Jesús Escudero

Agrobacterium colonizes plant cells via a gene transfer mechanism that results in plant tumorigenesis. Virulence (vi4 genes are transcriptionally activated in the bacteria by plant metabolites released from the wound site. Hence, it is believed that agrobacteria use injuries to facilitate their entrance into the host plant and that the wounded state is required for plant cell competence for Agr...

Journal: :Journal of bacteriology 1998
L E Stahl A Jacobs A N Binns

Agrobacterium tumefaciens causes crown gall disease by transferring oncogenic, single-stranded DNA (T strand), covalently attached to the VirD2 protein, across the bacterial envelope into plant cells where its expression results in tumor formation. The single-stranded DNA binding protein VirE2 is also transferred into the plant cell, though the location at which VirE2 interacts with the T stran...

Journal: :Glycobiology 2013
Thomas Geske Katharina Vom Dorp Peter Dörmann Georg Hölzl

Phosphate deficiency is characteristic for many natural habitats, resulting in different physiological responses in plants and bacteria including the replacement of phospholipids by glycolipids and other phosphorous-free lipids. The plant pathogenic bacterium Agrobacterium tumefaciens, which is free of glycolipids under full nutrition, harbors an open reading frame (ORF) coding for a processive...

2016
Venkidasamy Baskar Baniekal H. Gangadhar Se Won Park Shivraj Hariram Nile

The present study aims to investigate the numerous factors influencing Agrobacterium tumefaciens-mediated genetic transformation of Chinese cabbage (Brassica rapa ssp. pekinensis). Factors affecting transformation efficiency, such as age of explants, Agrobacterium concentration, and effect of acetosyringone, pre-cultivation, infection and co-cultivation time of Agrobacterium were examined. The ...

2017
Rex Frimpong Anane Huifang Sun Lamei Zhao Le Wang Chun Lin Zichao Mao

BACKGROUND This work evaluated the improvement of curdlan production of Agrobacterium sp. ATCC 31749 by using culture medium containing juice of discarded bottom part of green Asparagus spear (MJDA). Curdlan production was carried out using Agrobacterium sp. ATCC 31749 in flasks with different volumes of MJDA and its non-juice-adding control (CK) incubated in shaker at 30 °C, 200 rpm rotation f...

Journal: :The Plant journal : for cell and molecular biology 1998
S J Clough A F Bent

The Agrobacterium vacuum infiltration method has made it possible to transform Arabidopsis thaliana without plant tissue culture or regeneration. In the present study, this method was evaluated and a substantially modified transformation method was developed. The labor-intensive vacuum infiltration process was eliminated in favor of simple dipping of developing floral tissues into a solution co...

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