Abstract A controlled environment and composition of the compost used for commercial production Agaricus bisporus can allow fruiting bodies with increased nutritional value to be obtained. The present study aimed first investigate content bioelements in A. harvested from three flushes cultivation estimate which elements are accumulated most effectively by determination bioconcentration factor (...

4-(Hydroxymethyl)benzenediazonium tetrafluoroborate was administered as 26 weekly s.c. injections of 50 microgram/g body weight to randomly bred Swiss mice. In addition, as a solvent control, sodium tetrafluoroborate was given as 26 weekly s.c. injections at 25 microgram/g body weight in 0.9% NaCl solution to another group of mice. The 4-(hydroxymethyl)benzenediazonium tetrofluoroborate treatme...

The pattern of distribution of genetic markers among progeny of Agaricw bisporus, whether conventional mutants, electrophoretic isozyme variants or restriction fragment length polymorphisms, implies a combination of reduced recombination rates and preferential segregation of centromeres during meiosis. We have considered an alternative explanation, namely that meiosis may be an infrequent event...

This paper reports the inhibition kinetics of mushroom (Agaricus bisporus; J.E. Lange) polyphenol oxidase (PPO) with benzoic acid, sodium azide and sodium fluoride as inhibitors, and pyrocatechol as substrate. Mushroom PPO was significantly inhibited by all of the tested inhibitors. Diphenolase activity of mushroom PPO exhibited the competitive-type inhibition with benzoic acid as an inhibitor;...

Extracellular laccase (EC 1 .14.18.1) of the cultivated mushroom Agaricus bisporus was produced constitutively in defined or complex media. No enzyme induction was found after treatment with cycloheximide or with other potential inducers such as toluidine or xylidine. The enzyme was purified to homogeneity by ammonium sulphate precipitation, ion-exchange chromatography, gel filtration and affin...

A study was conducted to evaluate the effect of mushroom (Agaricus bisporus) and probiotics (Saccharomyces cerevisiae) on broiler performance. Day-old chicks (n=360) strain (Cobb 400) were divided randomly into eight groups. Each represented a treatment (45 birds/ treatment) with triplicate in completely randomized design. The experimental diets designed as, T1: control, T2: 0.4% powder, T3: 0....

Mushrooms contain multiple essential nutrients and health-promoting bioactive compounds, including the amino acid L-ergothioneine. Knowledge of chemical composition different mushroom varieties will aid research on their properties. We compared metabolomes fresh raw white button, crimini, portabella, lion’s mane, maitake, oyster, shiitake mushrooms using untargeted liquid chromatography mass sp...

This report describes an unusual substrate specificity associated with the y-glutamyltransferase present in sporophores of Agaricus bisporus, the mushroom of commerce in the United States. A study of the properties of such an enzyme from this organism was prompted by recent reports of the isolation and characterization of two unique 7-glutamyl derivatives, N(y-L(+)-glutamyl)-p-hydroxyaniline 1 ...

To characterize thermotolerance-related genes in Agaricus bisporus strain 02, we employed differential display PCR (DD-PCR) to analyze total RNA samples extracted from the mycelia grown at different temperatures. Two partial DNA fragments (023-11A and 023-11B) were cloned thus far, the expression of which was correlated with the culturing temperature. The sequences of the two DNA fragments were...