Recovery of adenosine triphosphate after myocardial ischemia is limited by the slow adenine nucleotide de novo synthesis and the availability of precursors of the nucleotide salvage pathways. We determined the adenine nucleotide de novo synthesis in the dog by infusion of [C]glycine and the acceleration of adenine nucleotide built up by intracoronary infusion of ribose together with [C]glycine ...

PURPOSE We determined the role of purine and pyrimidine nucleotides in erectile function in diabetic rats. MATERIALS AND METHODS A total of 60 adult male rats were divided into 2 groups, including 30 controls and 30 treated with streptozotocin (60 mg/kg) for 8 weeks to induce hyperglycemia. Changes in intracavernous pressure after intracrural injections of adenosine 5'triphosphate and adenosi...

The observed standard free energy change (AGibs) for the hydrolysis of the terminal pyrophosphate bond of ATP has been experimentally determined under physiological conditions using an entirely new set of reactions. The observed equilibrium constant (K,,bs) for the combined reactions of acetate kinase (EC 2.7.2.1) and phosphate acetyltransferase (EC 2.3.1.8) has been determined at 38”, pH 7.0, ...

Some of the physical and chemical properties of crystalline adenosine triphosphate-creatine transphosphorylase from rabbit muscle (1) have been previously described (2). In 1956, Friedberg reported on the amino acid composition of this enzyme (3). His data, however, were derived from analyses of only two samples hydrolyzed for the same time interval. Preliminary amino acid analyses from this la...

Purine derivatives like adenosine 5′-triphosphate (ATP) is the powerhouse of cell and essential to maintain cellular homeostasis activity. Besides this they also act as a chemical messenger when released into extracellular milieu because stress insult. The ATP (eATP) well its metabolite triggers purinergic signaling affecting various processes such cytokine chemokine production, immune function...

phosphate concentrations declined, indicating turnover of this compound. Incubation of LSI78Y cells in medium containing 0.5 ^M MMPR reduced intracellular concentrations of adenosine triphosphate, guanosine triphosphate, and adenosine diphosphate by about 70% and proliferation rates by about 30%. Upon transfer of such cells to M MPR-free medium, nucleotide levels and proliferation rates returne...