objective(s): vinblastine is antimitotic, anticancer medicine that disturbs normal microtubule formation and favours depolymerisation. structural study and finding the active site of vinblastine are the targets of this research.   materials and methods: vinblastine was optimized in vacuum and then in different solvents by density functional theory (dft) method. nuclear magnetic resonance (nmr) ...

In a shell and tube heat exchanger, the failure of tube-to-tubesheet welds results in high-pressure water jet which erodes the refractory in front of the tubesheet. Finite element method was employed to simulate the welding process and post weld heat treatment (PWHT) to find the factors affecting the failure in tube-to-tubesheet weldments. Residual stresses in two different geometries of tube-t...

We have evaluated the NMR shielding tensors for active site of oxidized ayurin Azurin is classified to atype I copper protein with ET functionality. We have computed NMR shielding tensor at .133LYP and IDlevels by usum 6-3IG basis set in the gas phase and in different solvents such as water, HMSO,Nitromethane, methanol, ethanol, acetone ,dicholoroethane. These solvents represent a wide range of...

The wild type Copia Gag precursor protein of Drosophila melanogaster expressed in Escherichia coli was shown to be processed autocatalytically to generate two daughter proteins with molecular masses of 33 and 23 kDa on SDS/PAGE. The active-site motif of aspartic proteinases, Asp-Ser-Gly, was present in the 23 kDa protein corresponding to the C-terminal half of the precursor protein. The coding ...

Arthrobotrysamerospora (ATCC 34468) produced glucoamylase in a semi-synthetic medium containing starch as a sole carbon source. Polyacrylamide gel electrophoresis of crude glucoamylase showed three isoenzymes. They were designated as glu I, glu II and glu III according to their electrophoretic mobility. These iso-glucoamylases were purified by column chromatography using DEAE-Sephadex A-50. The...

The relation between catalytic reactivities and metal/metal oxide ratios, as well the functions of metal oxides were investigated in CO2 hydrogenation reaction over highly active Cox(CoO)1–x catalysts operando. reactivity samples methanation improves with increased CoO concentration. Strikingly, sample highest concentration CoO, i.e., Co0.2(CoO)0.8, shows activity at temperatures lower than 200...