Chemical composition of propolis has been reported to be phyto-geographical in nature. The aim this study was isolate and characterize chemical compounds from hexane ethyl acetate extract obtained Gboko. To compounds, subjected column chromatography by gradient elution using two solvent mixtures - hexane: acetate: methanol. structure the isolated compound established 1H-NMR further verification...

Despite its distance from the active site the flexible amino-terminal segment (NTS) in the β-domain of the plant sesquiterpene cyclase δ-cadinene synthase (DCS) is essential for active site closure and desolvation events during catalysis.

A simple and cost-effective method for the synthesis of sulfated β-cyclodextrin, one most widely used chiral mobile phase additives, using sulfamic acid as a sulfonating agent has been described. The was optimized, synthesized product characterized by spectroscopic, size-exclusion chromatographic, thermal, microscopic methods compared to marketed Sigma Aldrich β-cyclodextrin. β-Cyclodextrin, hy...

Suitably configured allyl ethers of unsaturated cyclitols act as substrates β-glycosidases, reacting via allylic cation transition states. Incorporation halogens at the vinylic position these carbasugars, along with an activated leaving group, generates potent inactivators β-glycosidases. Enzymatic turnover halogenated (F, Cl, Br) displayed a counter-intuitive trend wherein most electronegative...

Although clinical studies have evaluated several MEK1/2 inhibitors, it is unlikely that MEK1/2 inhibitors will be studied clinically. BRAF mutations have been proposed as a responder marker of MEK1/2 inhibitors in a preclinical study. However, current clinical approaches focusing on BRAF mutations have shown only moderate sensitivity of MEK1/2 inhibitors. This has led to insufficient support fo...

Tal-effector nucleases (TALENs) are engineered proteins that can stimulate precise genome editing through specific DNA double-strand breaks. Sickle cell disease and β-thalassemia are common genetic disorders caused by mutations in β-globin, and we engineered a pair of highly active TALENs that induce modification of 54% of human β-globin alleles near the site of the sickle mutation. These TALEN...

Escherichia coli (328 isolates), Klebsiella pneumoniae (296), Klebsiella oxytoca (44), and Proteus mirabilis (33) isolates collected during 2012 from the nine U.S. census regions and displaying extended-spectrum-β-lactamase (ESBL) phenotypes were evaluated for the presence of β-lactamase genes, and antimicrobial susceptibility profiles were analyzed. The highest ESBL rates were noted for K. pne...

In bronchopulmonary dysplasia (BPD), alveolar septa are thickened with collagen and α-smooth muscle actin-, transforming growth factor (TGF)-β-positive myofibroblasts. We examined the biochemical mechanisms underlying myofibroblastic differentiation, focusing on the role of glycogen synthase kinase-3β (GSK-3β)/β-catenin signaling pathway. In the cytoplasm, β-catenin is phosphorylated on the NH(...

The interest in dynamic processes on networks is steadily rising recent years. In this paper, we consider the (α,β)-Threshold Network Dynamics ((α,β)-Dynamics), where α≤β, which only structural dynamics (edge of network) are allowed, guided by local threshold rules executed each node. particular, discrete round t, active pair nodes u and v, computes a value E(u,v) (the potential pair) as functi...

OBJECTIVE To examine the synergistic effect of interaction between perceived health and social activity on depressive symptoms. METHODS We investigated whether the interaction between perceived health and social activity has a synergistic effect on depressive symptoms in the middle-aged and elderly using data from 6590 respondents aged 45 and older in the Korean Longitudinal Study on Aging (K...