Oligonucleotide-directed triplex formation within upstream regulatory sequences is envisioned as a potential tool for gene inhibition. However, this approach requires that triple helix-forming oligonucleotides are chemically modified, so that the triplex is stable under physiological conditions. Here, we have compared several chemical modifications of an oligonucleotide, targeted to a natural 1...

Type II DNA topoisomerases are targets of acridine drugs. Nine mutations conferring resistance to acridines were obtained by forced molecular evolution, using methyl N-(4'-(9-acridinylamino)-3-methoxy-phenyl) methane sulfonamide (mAMSA), methyl N-(4'-(9-acridinylamino)-2-methoxy-phenyl) carbamate hydrochloride (mAMCA), methyl N-(4'-(9-acridinylamino)-phenyl) carbamate hydrochloride (AMCA), and ...

Background: Laboratory diagnosis of malaria is currently recommended for the confirmation of the disease before management. The two most common techniques in use for the diagnosis of malaria parasite in Sudan are Giemsa stained smears and Rapid diagnostic tests(RDTs). This study was carried out to compare microscopy ( Giemsa and Acridine orange stain ) and RDTs as effective tools for the diagno...

Carbamazepine is a widely used anticonvulsive agent. Its metabolic pathway leads not only to the major active metabolite, carbamazepine-10,11-epoxide, but also to minor terminal metabolites such as iminostilbene and acridine. Carbamazepine is usually well-tolerated, but it may lead to rare, but serious, hypersensitive reactions associated with hypereosinophilia. The mechanisms of hypersensitivi...

R E C E N T studies have distinguished two types of reversible mutations with respect to the type of change in the DNA molecule, in viruses and probably also in higher organisms (BRENNER, BARNETT, CRICK, and ORGEL 1961; MAGNI, personal communication). One type, involving substitution of one base for another and including transitions and possibly transversions, will be referred to here as “subst...

cases are reported below :? Case 1.?M. C. M., Hindu male, aged 28 years. Complaints of frequent attacks of diarrhoea for the last five years, indigestion, loss of appetite, flatulence, vague pain in the abdomen. Had been treated by his village doctor with emetine but had no improvement. On palpation of the abdomen nothing could be detected. Stool was examined, and numerous trophic forms of Giar...

1 Institute for Science and Public Policy, The University of Oklahoma, Norman, Oklahoma, USA 2 Department of Zoology, National Food Safety and Toxicology Center and Center for Integrative Toxicology, Michigan State University, East Lansing, Michigan, USA 3 Department of Forest Science, Oregon State University, Corvallis, Oregon, USA 4 Department of History, The Ohio State University, Columbus, ...

Anti-apoptotic proteins Bcl-2 and Bcl-xL could block autophagy by binding to Beclin 1 protein, an essential inducer of autophagy. Compounds mimicking Beclin 1 might be able to disrupt Bcl-xL/2-Beclin 1 interaction, free out Beclin 1, and thus trigger autophagy. In order to identify small molecule Beclin 1 mimetics, a fluorescence polarization-based high-throughput screening of 50,316 compounds ...