A rapid and sensitive HPLC method was developed for determination of losartan in plasma. Losartan was extracted from plasma by a two-step extraction procedure using chloroform as extracting solvent in acidic medium. HPLC analysis was performed on a cyano reversed-phase column using phosphate buffer (pH 4.3), acetonitrile (750:250, v/v) as mobile phase with a flow rate of 0.9 mL/min. Sodium dicl...

The preparation and applicability of solid phase microextraction (SPME) fibers coated with a sol-gel organically modified silica based on 3-aminopropyltrimethoxysilane and polydimethylsiloxane (APTMS/PDMS) are described here. Micrographs of the coated fibers revealed a rugous surface; the thickness of the coating was estimated to be less than 30 microm. The APTMS/PDMS fibers were tested with sy...

High molecular weight amines, quaternary salts etc. have the potential of removing heavy metal ions including the toxic ones at low concentrations. In the present work an attempt has been made to extract Cr(VI) through emulsion liquid surfactant membrane (or, emulsion liquid membrane-ELM) from it’s acidic solution using alamine-336 and caustic soda as extractant and striping reagents respective...

A collaborative trial was conducted to determine the performance characteristics of an analytical method for the quantification of inorganic arsenic (iAs) in food. The method is based on (i) solubilisation of the protein matrix with concentrated hydrochloric acid to denature proteins and allow the release of all arsenic species into solution, and (ii) subsequent extraction of the inorganic arse...

1:1 and 1:2 complexes of americium(III) with a hydrophilic anionic SO3-Ph-BTP4- ligand were detected in acidic aqueous nitrate solutions by a solvent extraction method. The determined conditional stability constants of these complexes, logβ1 = 4.35 ± 0.07 and logβ2 = 7.67 ± 0.06, related to 1 M aqueous solutions, are much lower than the literature values for the analogous curium species, determ...

The separation of tetracyclines (TCs) using reversed-phase liquid chromatography (LC) is proposed. The use of an amide-based stationary phase prevents the interaction of tetracyclines with the residual silanol groups and thus avoids the appearance of tailed peaks. Detection was based on using an UV spectrophotometer and gradient elution with acetonitrile-oxalic acid as mobile phase permitted go...

biosorption of acidic dyes using the live fern azolla filliculoides was studied in a discontinuous system. dye parameters, dye initial concentration and contact time were studied in temperature range of 25-30 ?c and ph=7. it was shown that increasing the 23initial concentration of dye and its contact time resulted in decreasing the dye taking quantity by the absorbent. also, type of dye has an ...

Tobacco has proven to be a promising alternative for the production of recombinant therapeutic proteins and offers numerous advantages over other plants as a host system. However, the recovery and purification steps needed to obtain a protein at high recovery and purity have not been well investigated. In this study, a process was developed to purify a model acidic protein, recombinant beta-glu...

In this work, the ability of Na(2)SO(4) and ionic liquids to induce the formation of acidic aqueous two-phase systems (ATPS) is investigated. Ternary phase diagrams, tie lines, and tie-line lengths for several systems were determined and reported at 298 K and atmospheric pressure. It is here shown that among the ionic liquids studied only those containing long alkyl side chains at the ions and/...