An intestinal perfusion technique has been used in normal human subjects to investigate the influence that starter protein composition and hydrolysis procedure have on absorption of amino acid residues from partial enzymic hydrolysates of whole protein. Five starter proteins were studied. Three (egg albumin, casein/soy/lactalbumin, and lactalbumin) were hydrolysed by papain, a second lactalbumi...

Understanding mechanisms of (+)-anti-BPDE detoxification is crucial for combating its mutagenic and potent carcinogenic action. However, energetic-structural correlations of reaction intermediates and transition states during detoxification via hydrolysis are poorly understood. To gain mechanistic insight we have computationally characterized intermediate and transition species associated with ...

protein recovery under sonication treatment and autolysis, also protein hydrolysis progress during enzymatic hydrolysis (using trypsin and chymotrypsin) and autolysis (using endogenous enzymes) were investigated in saccharomyces cerevisiae and kluyveromyces marxianus. crude protein content of dried yeast cells were 53.22% and 45.6% for s.cerevisiae and k.marxianus, respectively. after 96 hrs of...

This study was aimed at explanation of evolution process bound phenolics from IDF through structural modifications after alkaline hydrolysis along with free and functional characteristics. SEM results depicted that exhibited denser, more compact tubular shape whereas, the surface features alkali-hydrolyzed residue clear fragmentation, wrinkling porosity on outside surfaces. Microstructure chang...

A new procedure for analyses for tryptophan in proteins and glycoproteins is described. The method utilizes ptoluenesulfonic acid as the catalyst for hydrolysis and the amino acid analyzer for the quantitative estimation of tryptophan. The hydrolysis of proteins is carried out in 3 N p-toluenesulfonic acid containing 0.2 % 3-(Z-aminoethyl)indole in evacuated sealed tubes at 110” for 22, 48, and...

The hydrolysis of potassium organotrifluoroborate (RBF(3)K) reagents to the corresponding boronic acids (RB(OH)(2)) has been studied in the context of their application in Suzuki-Miyaura coupling. The "slow release" strategy in such SM couplings is only viable if there is an appropriate gearing of the hydrolysis rate of the RBF(3)K reagent with the rate of catalytic turnover. In such cases, the...

Henriques and Gjaldbaek,l in an article devoted chiefly to a study of the above subject with the form01 titration, find that, when proteins are hydrolyzed with acid, both amino nitrogen and ammonia increase up to a certain point, at which the amino nitrogen attains its maximum. When hydrolysis is continued beyond this point, a transformation of amino nitrogen into ammonia follows, indicating a ...

Methanolysis of intervertebral disc samples at 600 was largely hydrolysis as shown by the absence of methylated derivatives on chromatograms of the reaction products. Extent of the reaction increased with acid strength; the products were the same. Maximum solubilization was about 80% and 50% for hyaline cartilage plate and nucleus pulposus samples, respectively. The results of aqueous acid (hyd...

BACKGROUND Acid hydrolyzed wheat proteins (HWPs) are used in the food and cosmetic industry as emulsifiers. Cases of severe food allergic reactions caused by HWPs have been reported. Recent data suggest that these reactions are caused by HWPs produced by acid hydrolysis. OBJECTIVES To examine the sensitizing capacity of gluten proteins per se when altered by acid or enzymatic hydrolysis relat...

microbial phytases were applied mainly to animal and human foodstuffs in order to improvemineral bioavailability and food processing. in addition, phytases have potentialbiotechnological application in various other fields, such as environmental protection,aquaculture and agriculture. bacillus sp. dm12, an isolate from a hot spring, produces phytase,which catalyzes the hydrolysis of phytic acid...