The beta-lactamase gene from Streptomyces fradiae Y59 was cloned and sequenced. To determine which amino acid residues are critical in binding activity to blue dextran, chimera beta-lactamases were constructed and their binding abilities were determined. The results suggested that blue dextran binding may depend more on overall conformation of about two-thirds of the beta-lactamase molecule fro...

Preliminary to a study of benign appendageal tumors of the skin, the results of which will be published separately, the following study of normal apocrine and eccrine glands was undertaken. The use of the periodic acid-Schiff (PAS) and Alcian Blue (AB) stains separately on tissue sections and the clarification of the two color reactions of the PAS stain by Alcian Blue (1) has lead to a better h...

In the previous communication (l), it was shown that the free energy of the bivalent fumarate ion at 25” is -144,630 calories. In the present communication the measurement of the equilibrium at 25” between fumaric acid and malic acid in the presence of fumarase is reported, and from the values obtained computations are made of the free energy and heat of formation of the bivalent I-malate ion a...

The stability of an emulsified liquid membrane composed of Span80 as a surfactant, D2EHPA as an extractant and sulfuric acid as an internal phase was first studied according to different diluents and many operating parameters using the Plackett-Burman design of experiments. Then the removal of methylene blue from an aqueous solution has been carried out using this emulsified liquid membrane at ...

This work proposes the concept of ratiometric electrochemical proximity assay (REPA), which can be used for one-step, highly sensitive and selective detection of protein. The assay strategy was achieved on a sensing interface that was formed by hybridization of methylene blue (MB)-labeled antibody-DNA probe (MB-DNA1-Ab1) with ferrocene (Fc)-labeled DNA capture probe (Fc-P) modified gold electro...

Many fluorescent proteins are currently available for biological spectroscopy and imaging measurements, allowing a wide range of biochemical and biophysical processes and interactions to be studied at various length scales. However, in applications where a small fluorescence reporter is required or desirable, the choice of fluorophores is rather limited. As such, continued effort has been devot...

Since Steedman ( 1950) has introduced it into histochemistry, alcian blue has been advocated to reveal a staining selectivity which depends upon the presence of acidic groups in mucopolysaccharides (Mowry, 1956; Pearse, 1960). As a plausible ground of this concept the fact has been presented that the alcian blue staining of polysaccharides can be increased in intensity, when acidic groups are i...

Reaction of 2-(methyl-sulfan-yl)pyrimidin-4-amine with the 5-(methoxy-vinyl-idene) derivative of Meldrum's acid and subsequent heating of the product in Dowtherm fluid yielded the title compound, C(8)H(7)N(3)OS, which was proven to contain a bicyclic 4H-pyrimido[1,6-a]pyrimidine system. All non-H atoms of the mol-ecule are coplanar within 0.15 Å. The bond-length distribution in the bicyclic cor...

In this study, ryegrass straw agricultural residue (Lolium multiflorum L.) was employed as an adsorbent material to remove methylene blue (MB) dye from aqueous solutions. Four adsorbents were produced using phosphoric acid and pyrolysis activating agents. The samples analyzed with TGA, FTIR, SEM, XRD techniques. A rapid adsorption of the MB obtained treated 40% H3PO4, reaching equilibrium in 2 ...