The involvement of phospholipase A2 (PLA2) and fatty acid release in the regulation of sodium-dependent high-affinity choline uptake in rat brain was assessed in vitro through the use of the specific binding of 3H-hemicholinium-3 (3H-HCh-3). Addition of arachidonic acid and other unsaturated fatty acids to rat striatal membranes in vitro resulted in a dose-dependent, temperature-independent act...

Atrial-natriuretic-peptide (ANP) receptor, previously identified as a 140 kDa protein with a disulphide-linked homodimeric structure, was purified from bovine lung by (NH4)2SO4 fractionation and affinity chromatography on ANP-Affi-Gel 10. The purified receptor had a binding capacity of 4.2 nmol of ANP/mg of protein and an affinity constant of 6.5 pM. The isoelectric point of the receptor was 5....

The valency of quantum dot nanoparticles conjugated with biomolecules is closely related to their performance in cell tagging, tracking, and imaging experiments. Commercially available streptavidin conjugates (SAv QDs) are the most commonly used tool for preparing QD-biomolecule conjugates. The fluorescence quenching of biotin-4-fluorscein (B4F) provides a straightforward assay to quantify the ...

The ability of altered transfer ribonucleic acid to compete with binding of [14C]aminoacyland [l*C]tRNA was used as an estimate of its capacity to bind to ribosomes. Transfer ribonucleic acid with the terminal 3’-hydroxyl rA, ,C,A, and ,C,C,A enzymatically removed could bind to 70 S ribosomes with progressively lower efficiency; in contrast, binding to 30 S subunits was unimpaired. Transfer rib...

to study the effects of drought stress and foliar application with salicylic acid on yield andit’s components and percentage of essential oil of nigella sativa l., a field experiment was conducted as split plot based on randomized complete block design with three replications at research farm of shahid bahonar university of kerman during growing season of 2013- 2014. the main factor was drought...

Cadmium (Cd) is a highly toxic heavy metal, wide occupational and an environmental pollutant, affecting human health. Probiotics especially lactic acid bacteria (LAB) have the capacity to bind, remove and to decrease tissue cadmium levels. The objective was to evaluate the potency of Cd binding capacity, antioxidative properties of probiotic bacteria against cadmium in vitro and its probable de...

zinc ions are present in different types of industrial effluents, being responsible for environmental pollution. biosorption is a process in which solids of natural origin are employed for binding heavy metals. it is a promising alternative method to treat heavy metal wastes mainly because of high metal binding capacity. the effect of some important parameters on sorption capacity of cyanobacte...

The severity of SARS-CoV-2 infection is highly variable and yet the molecular basis for this effect remains elusive. One potential contribution are differences in glycosylation target human cells, particularly as has capacity to bind sialic acid which a common, variable, terminal modification glycans. viral spike glycoprotein (S) cellular receptor, angiotensin-converting enzyme 2 (ACE2) both de...

In this study twenty-four male and female one-humped and crossbred (Camelus dromedarius and Camelus bactrianus) camel meat were evaluated during two fattening periods (6 and 9 months). The characteristics of camel meat and fatty acid profile in different parts of body (leg, shoulder, loin and neck) were measured. The results indicated that the pH ultimate of crossbred...