A concept of tandem driven dynamic self-inhibition is demonstrated through dynamic inhibitors of acetylcholinesterase (AChE) using reversible transthiolesterification.

When Robert Laskowski, MD, president and CEO of Christiana Care Health System, Wilmington, Del., and an ACHE Member, speaks to new employees as part of their orientation, he talks about the organization’s mission, commonly referred to as the Christiana Care Way. It is a framework, a compass if you will, that gets employees and the institution on the right path—the way—and keeps them there: “We ...

We introduce a one-step histochemical method with cobalt as the precipitating agent for ferrocyanide for the light microscopic demonstration of acetylcholinesterase activity. This method was used to demonstrate acetylcholinesterase in normal cortical fibers and neurons, as well as pathological elements such as plaques and tangles. This procedure can also be easily combined with immunohistochemi...

The synthesis, kinetic, and spectral characterization of (7-nitrobenz-2-oxa-1,3-diazole)aminoethyl and (7nitrobenz-2-oxa1,3-diazole)aminopentyl methylphosphonofluoridate are described. These homologous organophosphorous agents contain the environmentally sensitive 7-nitrobenz-2-oxa1,3-diazole chromophore. They inhibit acetylcholinesterase from Torpedo at rates exceeding lo’ M” min” to form l...

Up to the present, Alzheimer’s disease (AD), a dementia, is believed to be incurable; even its cause has not been documented. A few hypotheses have been postulated, however; among these are the destruction of acetylcholine by the enzyme acetylcholineesterase, the accumulation of amyloid beta plaque, and peroxidation of the brain cells. Since the disease was unknown in the olden days in Thailand...

Two structurally distinct molecular forms of acetylcholinesterase are found in the electric organs of Torpedo californica. One form is dimensionally asymmetric and composed of heterologous subunits. The other form is hydrophobic and composed of homologous subunits. Sequence-specific antibodies were raised against a synthetic peptide corresponding to the COOH-terminal region (Lys560-Leu575) of t...