We have constructed a synthetic ecosystem consisting of two Escherichia coli populations, which communicate bi-directionally through quorum sensing and regulate each other's gene expression and survival via engineered gene circuits. Our synthetic ecosystem resembles canonical predator-prey systems in terms of logic and dynamics. The predator cells kill the prey by inducing expression of a kille...

Abstract Transgenic plants that express double-stranded RNA (dsRNA) targeting vital insect genes have recently emerged as a valuable new tool for pest control. In this study, tobacco were transformed to produce dsRNA Sl 102 gene is involved in the immune response of Spodoptera littoralis larvae, serious lepidopteran several crops. Experimental larvae reared on transgenic lines showed (1) strong...

Thymidine incorporation by HEp-2 cells was reduced 10-fold by 2.5 mM hydroxyurea (HU). The DNA in lysates from untreated cells sedimented through alkaline sucrose gradients as an aggregate plus a smaller component which could be chased into the aggregate. Only small DNA (ca. 47 S) was synthesized in nuclei when cells were incubated with HU. HU inhibition was reversible up-to 24 hr. Removal of i...

A terbium complex has been designed that may be used to sensitise formation of singlet oxygen (phi(em) 12%), allowing the creation of a ratiometric sensory system for pO(2) (versus the insensitive Eu analogue) and the definition of a reactive probe that can be used to cause local damage to cells loaded with the complex and exposed to laser excitation.

Reactive oxygen species (ROS) are widely believed to kill malarial parasites. C57BL/6 mice injected with P. berghei inocula incubated with supraphysiological doses of NO (< or =150 microM) or with peroxynitrite (220 microM), however, exhibited parasitemia similar to that seen with those given control inocula, and there was no difference in disease development. Only treatment of inocula with NO ...

osteomyelitis is an inflammatory infectious condition of bones, occurring either acutely or chronically. the clinical course of the disease leads to destructive or sclerosing patterns of the involved bone. this report presents a case of chronic focal sclerosing osteomyelitis in a 19-year-old male with a history of an uncontrolled convulsive condition. the lesion was first diagnosed as an osteob...

the purpose of this study was to determine the seasonal changes of vital signs (body temperature, pulse rate and blood pressure) in relation to the working thermal conditions. in this study a total number of 202 permanent workers of bakeries were studied during summer and winter. the results showed the body temperature and pulse rate raised more in summer than in winter in all workers except wh...