A quantitative one-step SYBR Green I-based reverse transcription (RT)-PCR system was developed for the detection and differentiation of four different dengue virus serotypes in acute-phase serum samples. A set of group- and serotype-specific primer pairs was designed against conserved sequences in the core region and evaluated for clinical diagnosis. A linear relationship was obtained between t...

The potential leptospiral infection hazard in the use of vaccines prepared from canine kidney monolayer cultures was studied. Cell cultures were prepared from kidneys of dogs experimentally infected with Leptospira serotype canicola. Viable leptospires were found in kidney cell suspensions at the time of seeding, surviving trypsinization either at room temperature for approximately 2 hr or over...

The objective of this study was to assess the influence of specific factors on post-thaw development of mouse cryopreserved morulae. Thawed morulae (n = 206) were randomly distributed between 10 treatment groups: medium alone control (CT), Vero (VR) cells, leukaemia inhibitory factor (1 ng/ml), interleukin-6 (1 ng/ml), transforming growth factor (TGF) alpha (2 ng/ml), epidermal growth factor (E...

Strains. The strains examined are all maintained in the Pasteur Culture Collection (PCC) and havenow been deposited in the American Type Culture Collection (ATCC). They are identified here by both PCC and ATCC strain numbers. Full strain histories, media employed for cultivation and the explanation of generic terminology are given by Rippka et al. (1979). An additional strain (Oscillatoria agar...

The volume of blood sampled for blood culture determines its sensitivity. We measured low mean blood volumes in submitted aerobic (8.38 ± 3.88 ml) and anaerobic (7.16 ± 3.83 ml) blood culture bottles. Educational seminars were held for phlebotomy teams, and renewed measurements thereafter revealed significantly higher blood volumes in submitted aerobic (9.77 ± 4.42 ml) and anaerobic (8.30 ± 3.6...

Strains. The strains examined are all maintained in the Pasteur Culture Collection (PCC) and havenow been deposited in the American Type Culture Collection (ATCC). They are identified here by both PCC and ATCC strain numbers. Full strain histories, media employed for cultivation and the explanation of generic terminology are given by Rippka et al. (1979). An additional strain (Oscillatoria agar...

Phytohemagglutinin (PHA) is a lectin, obtained from the red kidney bean that binds to the membranes of T-cells and stimulates metabolic activity, cell division, etc. The object of this research was the comparison between self made PHA (Indigenous) and imported commercial one, following conventional and High Resolution Cell Synchronization technique (HRCS) .From each blood sample of healthy indi...

Growth properties of cells cultured from primary mammary tumors of C3H mice have been analyzed. Cells were seeded at 2 different densities (1 X 10(5) and 5 X 10(5)/sq cm) and were supported with a culture fluid containing 10% fetal calf serum and 5 mug insulin per ml. Mitosis continued after confluence was achieved, but cells did not accumulated in the monolayer; rather, certain cells were rele...

Protein constructs and purification For purification of NFκB:IRF-7:IRF-3, a freshly transformed colony was transferred into 10 ml LB broth containing 100 μg/ml ampicillin and grown overnight at 37°C to saturation. This overnight culture was used to inoculate 1 liter LB broth containing 100 μg/ml ampicillin and grown at 37°C to an OD600 of about 0.5. The culture was then induced with 0.4 mM isop...

Recently, the ligand for the Mpl receptor (ML) was identified to be thrombopoietin, the principal regulator of megakaryocytopoiesis and thrombopoiesis. We examined the effects of ML, as a single factor or in combinations with early acting factors such as steel factor (SF), interleukin (IL)-3, IL-1, IL-6, and granulocyte colony-stimulating factor (G-CSF), on colony formation from primitive proge...