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Unlike general peroxidases, Pleurotus ostreatus MnP2 was reported to have a unique property of direct oxidization of high-molecular-weight compounds, such as Poly R-478 and RNase A. To elucidate the mechanism for oxidation of polymeric substrates by MnP2, a series of mutant enzymes were produced by using a homologous gene expression system, and their reactivities were characterized. A mutant en...
The British Columbia Center for Disease Control laboratory performs approximately 95% of all hepatitis C virus (HCV) antibody tests for the province's 4 million inhabitants. In 2002, the laboratory tested 96,000 specimens for anti-HCV antibodies, of which 4,800 (5%) were seroreactive and required confirmation of active infection. Although HCV RNA assays with a sensitivity of 50 IU/ml or less ar...
VPs (versatile peroxidases) sharing the functions of LiP (lignin peroxidase) and MnP (manganese peroxidase) have been described in basidiomycetous fungi Pleurotus and Bjerkandera. Despite the importance of this enzyme in polymer degradation, its reactivity with polymeric substrates remains poorly understood. In the present study, we first report that, unlike LiP, VP from Pleurotus ostreatus dir...
In this work, we studied the influence of the alkyl chain length in enzymatically oxidized gallates on the development of hydrophobicity on paper-based materials, and further correlated the obtained effect to the redox mechanism of the enzymatic treatment. Laccase (Lac) enzyme was used to oxidize various members of the gallate homologous series in the presence or not of lignosulfonates (SL) to ...
■ CONTENTS Nanoelectrodes 473 Extraordinary Properties of Nanoelectrodes 474 Nanoelectrode Fabrication 474 Nanoskiving 475 Lithographically Patterned Nanowire Electrodeposition 475 SWNT-Templated Nanowires and MCEM 476 Voltammetry and Double Layer Effects on Nanoelectrodes 476 Effect of Electrical Double Layer 476 Nanoparticle Electrochemistry 477 Monolayer Protected Clusters 477 Stochastic Nan...
Gummy stem blight is a major foliar disease of muskmelon (Cucumis melo L.). In this study, morphological characteristics and rDNA internal transcribed spacer (ITS) sequences were analyzed to identify the causal organism of this disease. Morphological examination of the Jeonbuk isolate revealed that the percentage of monoseptal conidia ranged from 0% to 10%, and the average length × width of the...
3 Martin Höhne, Johannes Lorscheider, Anna von Bardeleben, Matthias Dufner, M. 4 Antonia Scharf, Markus Gödel, Martin Helmstädter, Eva-Maria Schurek, Sibylle Zank, 5 Peter Gerke, Christine Kurschat, Sema Hayriye Sivritas, Elke Neumann-Haefelin, 6 Tobias B. Huber, H. Christian Reinhardt, Bernhard Schermer, Karl-Friedrich 7 Fischbach, Thomas Benzing 8 9 Renal Division, Department of Medicine and ...
This report summarizes results relative to seasonal changes in cholesterol, and provides alternative results to a report based on winter-summer differences in cholesterol (sea01d01.doc). We report descriptive data on the subjects in the seasonal cholesterol study by gender, and within gender, by age (< 50 or 50+). We also include results for variables corresponding to BMI, waist, hip and waist:...
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