The properties of the specific binding of the muscarinic receptor ligands [3H]quinuclidinyl benzilate and N-[3H]methylscopolamine in rat brain were compared. The specific binding of both ligands was affected equally by heat, phospholipase A2 and trypsin. N-[3H]methylscopolamine labeled only a fraction of the total muscarinic receptors recognized by [3H]quinuclidinyl benzilate in different brain...

The Taxco mining district is a well-known international producer of silver, jewelry, and precious metal handicrafts. Inappropriate disposal wastes from anthropogenic activities have been deteriorating the hydric resources threatening inhabitants’ health, since they use springwater for human consumption domestic activities. A multi-tracer approach combining measurements hydrochemical data, trace...

An electron-transport-type host was synthesized using a benzo[4,5]furo[3,2-d]pyrimidine (BFP) core to develop high triplet energy host. The designed have both the tetraphenylsilyl blocking group and hole-transport-type carbazole group. In device application, used as an mixed with 3,3†-di(9H-carbazol-9-yl)-1,1†-biphenyl (mCBP) in blue phosphorescent organic light-emitting diodes (PhO-LEDs) doped...

Clubroot disease resistance has been introgressed from Brassica rapa into canola following different approaches including a B. napus × interspecific cross; however, the details of this cross are not available. To extend our knowledge for introgression to multiple pathotypes, we crossed clubroot-resistant turnip with susceptible and backcrossed F1′s canola. The backcross population was subjected...

The physicochemical properties of nuclear and cytosolic glucocorticoid-binding components from corticoid-sensitive (CS) and corticoid-resistant (CR) mouse lymphoma P1798 cells have been compared. Nuclei or cytosol fractions were prepared from lymphocytes that had been labeled at 37 or 4 degrees, respectively, with 30 nM [3H]triamcinolone acetonide ([3H]TA). [3H]TA was extracted with 0.6 M KCl, ...

Binding of [3H]serotonin (5-HT) to membranes prepared from Arctic charr brain homogenates was most consistent with a one-site model for [3H]5-HT binding, with KD and Bmax values of 5.7±0.3 nmol l−1 and 60.7±7.3 fmol mg−1 protein, respectively. Similarly, 5-HT displacement of [3H]5-HT was best explained by a monophasic model with an apparent Ki of 4.3±0.7 nmol l−1. The ability of a number of syn...

Proluminal movement of 3H-testosterone from peritubular space to intratubular fluids of the adult rat testis and epididymis was studied by using in vivo microperfusion and subsequent micropuncture of seminiferous tubules and caput epididymal tubules. Tubules were perifused with Minimum Essential Medium containing 3H-testosterone. To determine if androgen transport is saturable, 40, 80, 160, and...