the abilities of silybum marianum cell culture to biotransform benzaldehyde to benzyl alcohol in three culture systems were compared. callus cultures of silybum marianum were established from seedlings, and healthy suspensions and immobilized cultures grown on the murashige and skoog medium. s. marianum cells were immobilized in both agar beads and fiber cotton matrixes. benzaldehyde was fed to...

Apert syndrome is caused by mutations in fibroblast growth factor receptor 2 (Fgfr2) and is characterized by craniosynostosis and other skeletal abnormalities. The Apert syndrome Fgfr2+/S252W mouse model exhibits perinatal lethality. A 3D hydrogel culture model, derived from tissue engineering strategies, was used to extend the study of the effect of the Fgfr2+/S252W mutation in differentiating...

Normal pancreatic acinar cells are difficult to maintain on traditional plastic culture surfaces due to their physical properties of housing large quantities of digestive enzymes and the formation of intercellular tight junctions and gap junctions (Apte and Wilson 2005; Rukstalis et al., 2003). However, placing primary acinar cells within a 3-dimensional matrix (3D-culture) maintains the cells ...

Adipocytes are a major component of the bone marrow that can critically affect metastatic progression in bone. Understanding how the marrow fat cells influence growth, behavior, and survival of tumor cells requires utilization of in vitro cell systems that can closely mimic the physiological microenvironment. Herein, we present two new three-dimensional (3D) culture approaches to study adipocyt...

In this study, a novel three-dimensional fluffy PPy conductive fibrous scaffold (3D-cFSs) was fabricated by electrospinning technique combined with situ surface polymerization. Chemical compositions, morphology were characterized by fourier transform infrared (FTIR) and scanning electron microscopy (SEM). The results showed that the average diameter of PPy coated PLLA fibers in the 3D-cFSs was ...

Specific niches within the tumor bone marrow (BM) microenvironment afford a sanctuary for multiple myeloma (MM) clones due to stromal cell-tumor cell interactions, which confer survival advantage and drug resistance. Defining the sequelae of tumor cell interactions within the MM niches on an individualized basis may provide the rationale for personalized therapies. To mimic the MM niche, we her...

The development of novel culture systems that mimic the in vivo microenvironment may be beneficial for inducing the differentiation of stem cells and promoting liver function. In the present study, spheroid cultures and decellularized liver scaffolds (DLSs) were utilized to obtain differentiated hepatocyte‑like cells. Mouse bone marrow (BM)‑derived mesenchymal stem cells (MSCs) self‑aggregated ...

Resetting the peripheral clock and understanding the integration between the circadian rhythm and metabolic pathways are fundamental questions. To test whether insulin acts as a synchronizer for the hepatic clock by cell-autonomous mechanisms, the phase-resetting capabilities of insulin were investigated in cultured hepatic cells. We provide evidence that three-dimensional (3D) cell culture con...

It has been recognized that bystander effect is one of the key factors for radiobiological effects, particularly in low-dose region. Although <1% of cell nuclei were actually traversed by an alpha particle, 30% of the cells showed an increased frequency of sister chromatid exchanges at very low dose (0.31 mGy) of alpha particles exposed to a CHO culture dish [1]. Since then, a number of studies...

The in vivo function and phenotype of ovarian follicle cells are determined by many factors. When these cells are removed from the in vivo microenvironment and grown in a 2D in vitro environment, the function of the follicular cells is difficult to preserve. A collagen hydrogel was used to examine the hormone and oocyte maturation of ovary follicles in a 3D culture system. Ovarian follicles fro...