This study focuses on the phylogenetic relationships within the Polyopisthocotylea and Monopisthocotylea, two groups that are often grouped within the monogeneans, a group of disputed paraphyly. Phylogenetic analyses were conducted with multiple outgroups chosen according to two hypotheses, a paraphyletic Monogenea or a monophyletic Monogenea, and with three methods, namely maximum parsimony, n...

ABSTRACT The primates that inhabit the rainforest surrounding city of Manaus (Amazonas, Brazil) have long been recognised as potentially important reservoirs emerging and re-emerging infectious diseases (ERIDs). PCR amplification filarial sequences from wild-caught Simulium oyapockense has used to incriminate Amazon-region ERID bridge vectors by showing they had previously fed on non-human prim...

Ribosome-inactivating Proteins (RIPs) are a group of cytotoxin proteins that usually contain a RNA N-glycosidase domain, which irreversibly inactivates ribosome, thus inhibiting protein synthesis. During the past 14 years (1990-2004), the studies conducted in our laboratory had been focusing on the structure and enzymatic mechanism of several PIPs. Herein, we briefly described a summary of the ...

In physiologically nongrowing lymphocytes, the rate of entry of new ribosomal RNA (rRNA) molecules to the cytoplasm is only half the rate at which rRNA is synthesized in the nucleus, although there is no net nuclear accumulation of rRNA (6) . When total cell RNA is extracted from intact lymphocytes after moderately short labeling periods, the amount of radioactivity appearing in 18S rRNA is onl...

Fungi have unique properties and are used in many areas of agriculture industry because they can produce different enzymes. This study aims to the fungal diversity peat soil from Pontian Johor, Malaysia. The isolates were described on culture media a new medium called EVA identified using phenotypical characteristics molecular D1/D2 domain 28S large subunit ribosomal RNA (28S rRNA) ITS (ITS1-IT...

RNA integrity plays an important role in RNA studies because poor RNA quality may have a great impact on downstream methodologies. This study proposes a cost-effective, rapid, and sensitive method for determining RNA integrity based on capillary electrophoresis that utilizes a cyan light-emitted diode-induced fluorescence as a separation tool. The capillary was initially coated with 0.1% Poly(v...

The ribosomal protein L22 is a component of the 60S eukaryotic ribosomal subunit. As an RNA-binding protein, it has been shown to interact with both cellular and viral RNAs including 28S rRNA and the Epstein-Barr virus encoded RNA, EBER-1. L22 is localized to the cell nucleus where it accumulates in nucleoli. Although previous studies demonstrated that a specific amino acid sequence is required...