When excised barley roots were incubated in 0.01 to 0.1 mm KCl solutions containing 0.2 mm CaSO(4), there was a peak in the K(+) accumulation against concentration curve at 0.02 to 0.04 mm KCl. The peak in the K(+) accumulation curve was shifted to lower K(+) concentrations when Ca(2+) concentration was decreased and to higher K(+) concentrations when Ca(2+) concentration was increased. Increas...

After incubation in isotonic KCl, dog red blood cells can be separated by centrifugation into subgroups which assume different cell volumes and possess different transport characteristics. Those red cells which swell in isotonic KCl exhibit a higher permeability to K and possess a greater volume dependence for transport of K than those red cells which shrink. A high Na permeability characterize...

The protoplasm of Nitella forms a thin layer surrounding a large central vacuole filled with sap. At the inner surface of the protoplasm is a non-aqueous layer called Y and at the outer surface is a similar layer called X. At each of these layers there is a potential due to the diffusion of KCl in contact with the layer. We thus have P = P(X) + P(Y) in which P is the total potential, P(X) is th...

A halotolerant, non-spore-forming actinobacterium was isolated from a soil sample from the west of China. The strain, designated YIM 70093(T) (=CCTCC AA 001024(T)=DSM 44683(T)), comprised Gram-positive, non-motile, diphtheroid and irregular rods. It grew in 0-25 % KCl (KCl could be substituted by NaCl or MgCl(2).6H(2)O), with optimum growth at 10 % KCl, and its optimal pH and cultivation temper...

We report a combined experiment-theory study on low energy vibrational modes in fluorescence spectra of perylene-3,4,9,10-tetracarboxylic acid dianhydride (PTCDA) molecules. Using very low coverages, isolated molecules were adsorbed on terrace sites or at sites located at residual steps on (100) oriented alkali halide films (KCl and NaCl). The low energy modes couple to the optical transition o...

A 190-kDa centrosomal protein interacts with microtubules when Drosophila embryo extracts are passed over microtubule-affinity columns. We have obtained a partial cDNA clone that encodes this protein. Using a fusion protein produced from the clone, we have developed a novel immunoaffinity chromatography procedure that allows both the 190-kDa protein and a complex of proteins that associates wit...

The effect of various concentrations of CaCl2 and KCl on egg white proteins during isoelectric precipitation of ovomucin was investigated in this study. At low concentrations of CaCl2 (<50 mM), lysozyme was the major contaminant in the precipitated ovomucin, whereas ovalbumin was the predominant one at high concentrations (>or=100 mM). At 50 mM CaCl2 concentration, the concentrations of both ly...

A culture of Pseudomonas fluorescens which grew at a minimal a(w) of 0.97 in a glucose-salts broth adjusted to that level with NaCl, KCl, or glucose, and at a(w) 0.9725 with Na(2)SO(4), was adapted to grow at lower a(w) levels produced by these solutes. Adaptation to growth at a low a(w) produced by means of one solute did not necessarily result in growth at that a(w) when another solute was em...

Calli of soybean [Glycine max cv. Pershing] were grown on "MS" media supplemented with different levels of KCl and NaH2PO4 for 45 days. The effects of salt stress were evaluated on the production and accumulation of proline and growth content of calli. Proline content increased as growth occurred. The maximum amount of proline and the highest content of growth were observed in calli grown on me...

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