نتایج جستجو برای: 2 extender containing 5 mgml bsa bsa
تعداد نتایج: 3388744 فیلتر نتایج به سال:
Herein, 1F2, an anti-HER2 monoclonal antibody (mAb), was covalently coupled to the surface of 5-Fluorouracil (5-FU) loaded bovine serum albumin (BSA) nanoparticles. Concerning two different crosslinkers for conjugation of 1F2, Maleimide-poly (ethylene glycol)-Succinimidyl carbonate (Mal-PEG5000-NHS) was selected due to its higher conjugation efficiency (23±4 %) obtained in comparison to N-succi...
Thymus cells from 5- to 6-week-old normal (unimmunized) BALB/c mice showed an increased incorporation of [(3)H]thymidine in the presence of 2,4-dinitrophenyl-bovine serum albumin, fluorescein-bovine serum albumin, and bovine serum albumin (BSA) in tissue culture. The concentrations of antigen (BSA and haptenated proteins) required for stimulation were approximately 25- to 50-fold higher than th...
Experiments were conducted to determine the efficiency with which viable, morphologically normal bovine spermatozoa could be isolated using a discontinuous bovine serum albumin (BSA) gradient. In the first experiment, extended semen was layered on top of a BSA gradient (4% BSA over 10% BSA) contained in a 500-ml separatory funnel. When comparing 1, 3, 5, 7, 14 or 21 X 10(9) spermatozoa applied ...
BACKGROUND Bovine serum albumin nanoparticles loaded with isoniazid and rifampicin (INH-RFP-BSA-NPs) were prepared and their release characteristics were studied in vitro. MATERIAL AND METHODS The INH-RFP-BSA-NPs were prepared by a modified self-emulsion solvent diffusion method, with albumin and polylactic acid used as carriers and to form the nanoparticles structure. Transmission electron mic...
in this study, the binding of amlodipine (a ca ++ channel blocker) and arsenic (metalloid) to bovine serum albumin (bsa) was studied by equilibrium dialysis(ed) method in order to have an insight into their binding chemistry to bsa. free amlodipine concentration was increased due to addition of arsenic which reduced the binding of the compounds to bsa. however, the free fraction was not increas...
BACKGROUND: The classic model of estrogen action requires that the estrogen receptor (ER) activates gene expression by binding directly or indirectly to DNA. Recent studies, however, strongly suggest that ER can act through nongenomic signal transduction pathways and may be mediated by a membrane bound form of the ER. Estradiol covalently linked to membrane impermeable BSA (E2-BSA) has been wid...
Background: Real-time three-dimensional echocardiography (RT-3DE) could obtain ventricular volume and ejection fraction rapidly non-invasively without relying on morphology. This study aims to use RT-3DE evaluate the changes in biventricular systolic function children with septal defect (VSD) moderate severe pulmonary hypertension (PH) before surgery. Methods: In this 18 VSD PH (VSD + Group) he...
A technique was developed for preparing a novel material that consists of gold nanoparticles trapped within a fiber of unfolded proteins. These fibers are made in an aqueous solution that contains HAuCl4 and the protein, bovine serum albumin (BSA). By changing the ratio of gold to BSA in solution, two different types of outcomes are observed. At lower gold to BSA ratios (30-120), a purple solut...
Surfactant protein D (SP-D, CP4) is a collagenous surfactant-associated glycoprotein synthesized by lung type II epithelial cells. SP-D can be selectively and efficiently eluted from isolated rat surfactant with glucose, maltose, and certain other saccharides. We therefore examined the ability of the purified protein to interact with carbohydrates in vitro. Saccharide-substituted bovine serum a...
In this research, the optimum conditions for the separation of polyphenols from apple juice (AJ) and the separation of BSA from a BSA/water solution were found by looking at the enrichment ratio (ER) and recovery. A mixture of the AJ and BSA was also studied to see how the different conditions affect separation of either compound. For AJ only, maximum ER was achieved at a feed concentration of ...
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