Xenopus oocytes are a popular model system for studying Ca signaling. They endogenously express two kinds of Ca-activated Cl currents, I(Cl-1), and I(Cl-2). I(Cl-1) is activated by Ca released from internal stores and, with appropriate voltage protocols, by Ca influx. In contrast, I(Cl-2) activation is dependent on Ca influx. We are interested in understanding how these two different Cl channel...

The following gold(I) and silver(I) complexes of the tritertiary phosphine 1,1,1- tris(diphenylphosphinomethyl)ethane, tripod , have been synthesised: Au(3)(tripod)X(3) [X = Cl(1), Br(2), I(3)]; [Au(3)(tripod)(2)Cl(2)]Cl (4); Au(tripod)X [X = Br(5), I(6)]; Ag(3)(tripod) (NO(3))(4) (7), Ag(tripod)NO(3) (8). They were characterized by X-ray diffraction (complexes 2, 3 and 4), (31)P NMR spectrosco...

X-ray diffraction studies demonstrate that oxidative addition of SiH(4) to Ir(PPh(3))(2)(CO)Cl yields Ir(PPh(3))(2)(CO)(Cl)(SiH(3))H, which features a cis arrangement of the SiH(3) and H ligands in which H is located trans to CO, rather than trans to Cl as originally reported. (1)H NMR spectroscopic studies indicate that oxidative addition of GeH(4) to Ir(PPh(3))(2)(CO)Cl also occurs in a cis m...

The kinetics of three chlorinated free radical reactions with Cl(2) have been studied in direct time-resolved measurements. Radicals were produced in low initial concentrations by pulsed laser photolysis at 193 nm, and the subsequent decays of the radical concentrations were measured under pseudo-first-order conditions using photoionization mass spectrometer (PIMS). The bimolecular rate coeffic...

The crystal structure of the title compound, [Cd(2)Cl(2)(C(2)H(8)N(2))(4)]Cl(2), consists of binuclear centrosymmetric [Cd(2)(C(2)H(8)N(2))(4)Cl(2)](2+) cations and discrete chloride anions. The Cd(II) cation is coordinated by four N atoms of two ethyl-enediamine ligands and two symmetry-related chloride anions within a distorted CdN(4)Cl(2) octa-hedron. Two Cd(II) cations are connected by two ...

Direct stretch of beta1 integrin activates an outwardly rectifying, tamoxifen-sensitive Cl(-) current (Cl(-) SAC) via focal adhesion kinase (FAK) and/or Src. The characteristics of Cl(-) SAC resemble those of the volume-sensitive Cl(-) current, I(Cl,swell). Because myocyte stretch releases angiotensin II (AngII), which binds AT1 receptors (AT1R) and stimulates FAK and Src in an autocrine-paracr...

Adrenoceptor-mediated Cl- transport in cultured rabbit corneal endothelium was examined using a Cl(-)-sensitive fluorescent dye. The intracellular Cl- concentration ([Cl-]i) in the endothelial cells was estimated to be about 30 mM. Noradrenaline (0.001-0.1 mM) transiently decreased the [Cl-]i in a dose-dependent manner. Such a decrease in [Cl-]i was completely antagonized by pretreatment with t...