A previous study reported the cloning of a putative Prevotella nigrescens-specific DNA probe, Pn23, using random shotgun method. The present study evaluated the species-specificity of Pn23 for P. nigrescens using the clinical strains of Prevotella intermedia and P. nigrescens to develop Prevotella nigrescens-specific polymerase chain reaction (PCR) primers. The specificity was tested against 10...

The nosocomial spread of carbapenem-resistant Enterobacteriaceae (CRE) is a serious concern for health care facilities (1, 2), and environmental sources are being increasingly implicated (3– 6). Carbapenems can be used to select for CRE in environmental testing, but detection may be impacted when organisms are present in low numbers. Bacterial tolerance to carbapenems can increase as inoculum s...

Abstract Background In the United Arab Emirates, rotavirus A (RVA) remains a major cause of gastroenteritis in young children despite universal use vaccines. Monitoring genetic diversity circulating strains is essential to better understand disease burden. Although semi-nested multiplex-PCR popular method for RVA genotyping, some clinically significant may not be accurately identified by this m...

This study analyzed the enzymatic basis and molecular epidemiology of amoxicillin-clavulanate-resistant Escherichia coli isolated by the microbiology laboratory of a United States tertiary care hospital. From October 1998 to December 1999, all E. coli isolates were screened for ampicillin-sulbactam resistance. Of 283 isolates that tested resistant to ampicillin-sulbactam, 69 unique patient isol...

Abstract Staphylococcus aureus is a prevalent bacterial causative agent of bovine mastitis and human food illness by secreting enterotoxins. This study aims to identify the seven classic enterotoxin genes (SEA SEH) isolated from humans animals. A total 173 isolates 14 (goat cow) were amplified with 23S rRNA genes. The presence classical enterotoxins (sea-seh) was using multiplex polymerase chai...

Anomuran decapod crustaceans of the genus Galathea are widespread in rocky/gravelly subtidal areas around northwestern European coasts (Zariquiey Alvarez, 1968). Recent interest in the group has been stimulated by the growing commercial importance of squat lobsters as the target of an expanding European ¢shery. However, the biology and ecology of these animals remain poorly studied (De Grave & ...

BACKGROUND The purpose of the present study is to evaluate the prevalence, causative organisms, and visual acuity outcome in patients with culture-proven polymicrobial endophthalmitis. The method used in this study is the non-comparative, consecutive case series using a retrospective analysis of patients diagnosed with polymicrobial endophthalmitis for the period 2000 to 2010. RESULTS Polymic...

BACKGROUND Bacterial vaginosis (BV) is one of the leading causes of vaginal complaints among women of childbearing age. The role of Gardnerella vaginalis remains controversial due to its presence in healthy and BV-type vaginal microflora. The phenotypic and genotypic heterogeneity of G. vaginalis suggested the existence of strain variants linked with different health conditions. We sought to an...

Few literatures have elaborated on the clinical characteristics of children with thalassemia from low-prevalence areas. A retrospective analysis was conducted on children genetically confirmed with thalassemia at Seoul National University Children's Hospital in Korea. Nine children (1α thalassemia trait, 6β thalassemia minor, 2β thalassemia intermedia) were diagnosed with thalassemia at median ...