Platelet-activating factor (PAF) is a phospholipid with diverse biological functions mediated by a G protein-coupled receptor. We determined PAF receptor binding in lung membranes of four groups of perinatal lambs. Membrane protein (100 microg/ml) was incubated for 60 min at 30 degrees C with 0.5-24 nM of acetyl-[(3)H]PAF in 30 mM Tris buffer, pH 7.2, containing 0.25% BSA, 10 mM MgCl(2), and 12...

1. The thermic effect of a glucose load (50 g) was studied in ten control and eleven obese female subjects, using both direct and indirect calorimetry simultaneously. Experiments were done under conditions of thermal equilbrium (28 degrees and 30% relative humidity). 2. Thermal balance (heat production measured by indirect calorimetry minus heat losses measured directly) was negative in the con...

Maternal water restriction and the accompanying dehydration-induced anorexia may induce long-term physiological changes in offspring. We determined the impact of prenatal hypertonicity (Pre-Dehy) on offspring cardiovascular and osmoregulatory function. Pre-Dehy lambs were exposed to in utero hypernatremia (8- to 10-meq increase; 110-150 days of gestation) induced by maternal water restriction. ...

Pasteurella haemolytica is a major contributor to neonatal pneumonia in lambs; which continues to be a major problem. Experimentation was conducted to determine the efficacy of vaccinating pregnant ewes to reduce the incidence of pneumonia in newborn lambs. Vaccines utilized in this experimentation included three different commercial Pasteurella haemolytica vaccines intended for use in cattle a...

thirty six male chall lambs (four-month old and 26  ±1.5 kg body weight) were randomly assigned into four treatments (nine lambs per treatment) to study the effect of powerfeed® (a commercial mixture made from wheat processing by-product and wheat bran) on weight gain, feed intake, feed conversion ratio, serum sodium and potassium concentration and urine ph. lambs in control group were fed with...

Newborn lambs depend on their dams for passive transfer of immunoglobulins, primarily IgG, for protection from harmful pathogens until their own immunological defenses have developed. Previous studies have suggested that supplementation with Se results in a modest increase in IgG concentration in serum of newborn calves and lambs. To evaluate the effect of the Se source and supplementation rate...

Three-hour milk production measurements determined by machine milking at 3-d intervals throughout a 63-d lactation period were used to describe lactation curves for crossbred ewes lambing at 1 and 2 yr of age and rearing single and twin lambs. Age of ewe, type of rearing, and day of lactation affected (P < 0.05) milk production. Over the 63-d lactation, average daily milk production was 2.56 an...