The present investigation was carried out aiming to use the bioinformatics tools in order to identify and characterize, simple sequence repeats within the third Version of the date palm genome and develop a new SSR primers database. In addition single nucleotide polymorphisms (SNPs) that are located within the SSR flanking regions were recognized. Moreover, the pathways for the sequences assign...

PREMISE OF THE STUDY Microsatellite loci were isolated and characterized from Aconitum kusnezoffii (Ranunculaceae) to estimate male and female reproductive success and evaluate the effects of clonal growth on sexual reproduction. METHODS AND RESULTS A genomic enrichment approach was used to develop microsatellite markers. In three investigated A. kusnezoffii populations, a total of 19 microsa...

Grevillea iaspicula is a highly restricted species occupying limestone outcrops near Wee Jasper, New South Wales (Australia). To allow future assessment of the ecological processes shaping the structure of G. iaspicula populations, seven polymorphic microsatellite (SSR) loci were developed from a partial genomic library. Amplification and polymorphism of six previously derived G. macleayana SSR...

Nuclear microsatellite markers for Pungtungia herzi were developed using a combination of next-generation sequencing and Sanger sequencing. One hundred primer sets in the flanking region of dinucleotide and trinucleotide repeat motifs were designed and tested for efficiency in polymerase chain reaction amplification. Of these primer sets, 16 new markers (16%) were successfully amplified with un...

As part of this laboratory’s honeybee genetics and breeding program, DNA markers are needed for uses such as the identification of populations and species and marker-assisted breeding. In particular, DNA markers are needed to discriminate Varroa-mite-resistant Russian honey bees, imported from fareastern Russia, and their offspring (1), from the other honey bees found in the USA. I tried RAPDs,...

The genus Tulipa L. (Liliaceae) comprises about 100 species and Iran is considered as one of the main origins of tulips. In this research, genetic diversity and population structure of 27 wild populations of tulips collected from Iran were studied by 15 highly polymorphic and reproducible expressed sequenced tag-simple sequence repeat (EST-SSR) markers and 8 nucleotide binding site (NBS)-enzyme...

Simple sequence repeat (SSR) and Single Nucleotide Polymorphic (SNP), the two most robust markers for identifying rice varieties were compared for assessment of genetic diversity and population structure. Total 375 varieties of rice from various regions of India archived at the Indian National GeneBank, NBPGR, New Delhi, were analyzed using thirty six genetic markers, each of hypervariable SSR ...

BACKGROUND Timothy (Phleum pratense L.), a cool-season hexaploid perennial, is the most important forage grass species in Nordic countries. Earlier analyses of genetic diversity in a collection of 96 genebank accessions of timothy with SSR markers demonstrated high levels of diversity but could not resolve population structure. Therefore, we examined a subset of 51 accessions with REMAP markers...

Background: In plant breeding program to produce hybrid varieties, pair of male sterile and restorer fertility lines are required. Differentiation of lines possessing restorer fertility allele from the lines lacking it remove the need for the progeny test, and thus reducing the time and the cost in the hybrid production procedure. Canola breeding program in Iran has concentrate...

Pitaya (Hylocereus spp.) is a tropical fruit grown in Okinawa prefecture, Japan. In this study, we developed simple sequence repeat (SSR) markers to assess the genetic diversity of pitaya accessions collected prefecture. SSR were with next-generation sequencing, using Roche 454 GS FLX+ platform. Seventy primer sets tested for identification pitaya, including 50 AG motif and 20 AC SSRs. total, 1...