نتایج جستجو برای: 18s rrna genes
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Background: In fertile women, glycodelin and glutathione peroxidase 3 (GPx3) genes expression rises during the luteal phase, with a peak occurring during the implantation window. The expression of these genes decreases in women with myomas. To determine whether myomectomy would reverse glycodelin and GPx3 expression, we evaluated the transcript levels of these genes in the endometrium of patien...
Quantitative RT-PCR analysis. The following primers were used to amplify β-casein cDNA and 18S rRNA sequences: forward primer of the β-casein gene 5'-GCT CAG GCT CAA ACC ATC TC-3' and reverse primer 5'-TGT GGA AGG AAG GGT GCT AC-3'; forward primer of the 18S rRNA gene 5’-TCGGAACTGAGGCCATGATT-3’ and reverse primer 5’CCTCCGACTTTCGTTCTTGATT-3’. Fragments of β-casein gene and 18S rRNA were amplifie...
Six alternative hypotheses for the phylogenetic origin of Bilateria are evaluated by using complete 18S rRNA gene sequences for 52 taxa. These data suggest that there is little support for three of these hypotheses. Bilateria is not likely to be the sister group of Radiata or Ctenophora, nor is it likely that Bilateria gave rise to Cnidaria or Ctenophora. Instead, these data reveal a close rela...
Trichomonads are occasionally observed in the feces of dogs with diarrhea. On the basis of superficial morphological appearance, these infections have been attributed to opportunistic overgrowth of the commensal, Pentatrichomonas hominis. However, molecular characterization of canine trichomonads has never been reported. This study was performed to determine, by means of rRNA gene sequence anal...
The Saccharomyces cerevisiae RPS0A/B genes encode proteins of the 40S ribosomal subunit that are required for the maturation of 18S rRNA. We show here that the RPS0 genes interact genetically with TOM1. TOM1 encodes a member of the hect-domain-containing E3 ubiquitin-protein ligase family that is required for growth at elevated temperatures. Mutant alleles of the RPS0 and TOM1 genes have synerg...
Two new species of the genus Scottolana Huys, 2009, were collected from Korean waters. S. daecheonensis sp. nov. is morphologically similar to jasani Song, Kangtia, Khim, and Chullasorn, 2018, Yellow Sea, Korea, gomezi Nazari, Mirshamsi, Sari, Aliabadian, Martinez Arbizu, Bandar Abbas, Iran. However, it can clearly be distinguished its congeners by rostrum with two tiny lateral sensilla, 4-sege...
In the chicken, the nucleolus organizer regions, or sites of the genes encoding 18S, 5.8S, and 28S ribosomal RNA (rRNA), map to one pair of microchromosomes that can be identified by silver nitrate cytochemistry. This nucleolar organizer chromosome also contains the major histocompatibility complex. Chickens aneuploid for this chromosome have been identified and reproduced for over seven genera...
Cell culture is an effective technology for taxol production. This paper discusses the effect of Taxus cell cultures on the 18S rRNA gene sequences based on the phylogenetic analysis of cultured T. chinensis cells and related species. The phylogenetic tree is reconstructed using the maximum parsimony method and the relative rate test to test the hypothesis of a molecular clock. The phylogenetic...
Some generally accepted viewpoints on the phylogenetic relationships within the molluscan class Gastropoda are reassessed by comparing complete 18S rRNA sequences. Phylogenetic analyses were performed using the neighbor-joining and maximum parsimony methods. The previously suggested basal position of Archaeogastropoda, including Neritimorpha and Vetigastropoda, in the gastropod clade is confirm...
considering the importance of theileria equi infection in horse breeding industry and marketing, in the present study, we aimed to determine the prevalence of t. equi among dromedaries in central iran, where a considerable number of camels and horses are raised and equine theileriosis is quite prevalent. for this purpose, a total of 161 blood samples from camels were examined in terms of t. equ...
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